Mitochondrial protein import: precursor oxidation in a ternary complex with disulfide carrier and sulfhydryl oxidase
| Autor: | Judith M. Müller, Michael J. Baker, Agnieszka Chacinska, Kipros Gabriel, Michael T. Ryan, Diana Stojanovski, Bernard Guiard, Nikolaus Pfanner, Agnes Schulze-Specking, Dusanka Milenkovic |
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| Rok vydání: | 2008 |
| Předmět: |
Saccharomyces cerevisiae Proteins
Stereochemistry Mitochondrial intermembrane space Saccharomyces cerevisiae Biology Mitochondrial Membrane Transport Proteins Substrate Specificity Mitochondrial Proteins Mitochondrial membrane transport protein Report Mitochondrial Precursor Protein Import Complex Proteins Oxidoreductases Acting on Sulfur Group Donors Disulfides Protein Precursors Protein disulfide-isomerase Ternary complex Research Articles Oxidase test Endoplasmic reticulum Cell Biology Mitochondria Transport protein Protein Transport Biochemistry Multiprotein Complexes Mutation biology.protein Oxidoreductases Intermembrane space Oxidation-Reduction Protein Binding |
| Zdroj: | ResearcherID The Journal of Cell Biology |
| ISSN: | 1540-8140 0021-9525 |
| DOI: | 10.1083/jcb.200804095 |
| Popis: | The biogenesis of mitochondrial intermembrane space proteins depends on specific machinery that transfers disulfide bonds to precursor proteins. The machinery shares features with protein relays for disulfide bond formation in the bacterial periplasm and endoplasmic reticulum. A disulfide-generating enzyme/sulfhydryl oxidase oxidizes a disulfide carrier protein, which in turn transfers a disulfide to the substrate protein. Current views suggest that the disulfide carrier alternates between binding to the oxidase and the substrate. We have analyzed the cooperation of the disulfide relay components during import of precursors into mitochondria and identified a ternary complex of all three components. The ternary complex represents a transient and intermediate step in the oxidation of intermembrane space precursors, where the oxidase Erv1 promotes disulfide transfer to the precursor while both oxidase and precursor are associated with the disulfide carrier Mia40. |
| Databáze: | OpenAIRE |
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