Rapid method for the determination of capsaicin and dihydrocapsaicin in Gochujang using ultra-high-performance liquid chromatography
| Autor: | Hye-Young Seo, Ho-Moon Seog, Jaeho Ha, You-Shin Shim, Masahito Ito, Hiroaki Nakagawa, Hyun-Jin Nam |
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| Předmět: |
Pharmacology
Chromatography Chemistry Extraction (chemistry) Analytical chemistry High-performance liquid chromatography Analytical Chemistry Dihydrocapsaicin Solvent chemistry.chemical_compound Capsaicin Calibration Republic of Korea Solvents Environmental Chemistry Indicators and Reagents Particle size Methanol Ultra high performance Agronomy and Crop Science Chromatography High Pressure Liquid Food Analysis Food Science |
| Zdroj: | Scopus-Elsevier |
| Popis: | A sensitive and specific heating block method coupled with ultra-HPLC (u-HPLC) was developed for the analysis of capsaicin in Gochujang and validated by comparing with a conventional HPLC (AOAC Method 995.03). The method validation parameters yielded good results, including linearity, precision, accuracy, and recovery. The u-HPLC separation was performed on a reversed C18 column (50 2 mm id, particle size 2 m), followed by fluorescence detection (excitation 280 nm, emission 325 nm). Methanol was used as the extracting solvent, and the amount of sample taken was approximately 0.2 g; the optimum amount of extraction solvent and extraction time were 15 mL and 1 h, respectively. The recovery of capsaicin in Gochujang was more than 93, and the LOD and LOQ of the u-HPLC analysis were 0.05 and 0.16 g/g for capsaicin and 0.05 and 0.16 g/g for dihydrocapsaicin. The calibration graphs for capsaicin and dihydrocapsaicin were linear from 0.2 to 10.0 g/mL for u-HPLC. The interday and intraday precisions (RSD values) were |
| Databáze: | OpenAIRE |
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