Performance of GeneXpert ultra in the diagnosis of Tuberculous Cervical lymphadenitis in formalin fixed paraffin embedded tissues
| Autor: | Soumaya Rammeh, Meriam Ksentini, Helmi Mardassi, Emna Romdhane, Ilhem Boutiba Ben Boubake, Arfaoui A, Neira Dekhil, Asma Ferjani, Rim Lahiani, Mohamed-Ridha Barbouche, Mamia Ben Salah, Chaouki Benabdessalem, Soumaya Bchiri |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Microbiology (medical) Adult DNA Bacterial Male Pathology medicine.medical_specialty Tuberculosis Formalin fixed paraffin embedded Adolescent 030106 microbiology Immunology Tuberculosis Lymph Node Microbiology 03 medical and health sciences Young Adult Lymphadenitis Predictive Value of Tests mental disorders Medicine Humans Child Aged Retrospective Studies GeneXpert MTB/RIF Paraffin Embedding biology business.industry Mycobacterium tuberculosis Middle Aged Tuberculous cervical lymphadenitis medicine.disease biology.organism_classification Predictive value Paraffin embedded 030104 developmental biology Infectious Diseases Mycobacterium tuberculosis complex Ziehl–Neelsen stain Female Lymph Nodes business psychological phenomena and processes Neck |
| Zdroj: | Tuberculosis (Edinburgh, Scotland). 125 |
| ISSN: | 1873-281X |
| Popis: | The diagnosis of Tuberculous Cervical lymphadenitis (TCL) is challenging. The present study aimed to assess the performance of GeneXpert ultra (GXu) in the diagnosis of TCL on Formalin Fixed, Paraffin Embedded Tissues (FFPET). This study included 35 TCL cases confirmed by positive microbiology and/or positive GXu on Fresh Tissues (FT). The diagnostic performance parameters of GXu on FFPET were determined with reference to microbiology (positive Ziehl Neelsen and/or positive culture) and with reference to positive microbiology and/or positive GXu on FT. The GXu on FFPET was positive in 26/35 (74%) cases. With reference to positive ZN and or culture, the sensitivity, specificity, positive predictive value, and negative predictive value of GXu on FFPET were 63%, 100%, 100% and 71% respectively. With reference to positive microbiology and/or positive GXu on FT, these rates were 74%, 100%, 100% and 40% respectively. GXu on FFPET is a reliable tool for the detection of Mycobacterium tuberculosis complex particularly for cases where microbiological investigations have not been performed. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect