Helium postconditioning regulates expression of caveolin-1 and -3 and induces RISK pathway activation after ischaemia/reperfusion in cardiac tissue of rats
| Autor: | Renske Johanna Steenstra, Hemal H. Patel, Raphaela P. Kerindongo, Martin Albrecht, Markus W. Hollmann, Nina C. Weber, Benedikt Preckel, Coert J. Zuurbier, Gezina T. M. L. Oei, Moritz Flick |
|---|---|
| Přispěvatelé: | Anesthesiology |
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
Male
0301 basic medicine medicine.medical_specialty Caveolin 3 Caveolin 1 Myocardial Reperfusion Injury Biology Caveolins Helium 03 medical and health sciences 0302 clinical medicine Western blot 030202 anesthesiology Internal medicine Caveolae Caveolin medicine Animals RNA Messenger Rats Wistar Ischemic Postconditioning Protein kinase B Pharmacology Cardioprotection medicine.diagnostic_test Kinase Myocardium Rats Protein Transport 030104 developmental biology Endocrinology Gene Expression Regulation Biochemistry cardiovascular system Protein Kinases Signal Transduction |
| Zdroj: | European journal of pharmacology, 791, 718-725. Elsevier |
| ISSN: | 0014-2999 |
| Popis: | Caveolae, lipid enriched invaginations of the plasma membrane, are epicentres of cellular signal transduction. The structural proteins of caveolae, caveolins, regulate effector pathways in anaesthetic-induced cardioprotection, including the RISK pathway. Helium (He) postconditioning (HePoc) is known to mimic anaesthetic conditioning and to prevent damage from myocardial infarction. We hypothesize that HePoc regulates caveolin-1 and caveolin-3 (Cav-1 and Cav-3) expression in the rat heart and activates the RISK pathway. Male Wistar rats (n=8, each group) were subjected to 25min of cardiac ischaemia followed by reperfusion (I/R) for 5, 15 or 30min (I/R 5/15/30). The HePoc groups underwent I/R with 70% helium ventilation during reperfusion (IR+He 5/15/30min). Sham animals received surgical treatment without I/R. After each protocol blood and hearts were retrieved. Tissue was obtained from the area-at-risk (AAR) and non-area-at-risk (NAAR) and processed for western blot analyses and reverse-transcription-real-time-polymerase-chain-reaction (RT-qPCR). Protein analyses revealed increased amounts of Cav-1 and Cav-3 in the membrane of I/R+He15 (AAR: Cav-1, P |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect