Increased thymic output in HIV-negative patients after antiretroviral therapy
| Autor: | Joel G.R. Weaver, Andrew D. Badley, David J. McKean, Catherine J. Huntoon, Daniel B. Graham, Nanci Hawley, Michael P. Bell |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
Adult
T cell Immunology Recent Thymic Emigrant Receptors Antigen T-Cell Apoptosis HIV Infections Thymus Gland Biology Immunophenotyping Mice Antiretroviral Therapy Highly Active HIV Seronegativity medicine Immunology and Allergy HIV Protease Inhibitor Animals Humans Protease inhibitor (pharmacology) T-cell receptor virus diseases Middle Aged Thymocyte Infectious Diseases Nelfinavir medicine.anatomical_structure Case-Control Studies Leukocytes Mononuclear Central tolerance medicine.drug |
| Zdroj: | AIDS (London, England). 19(14) |
| ISSN: | 0269-9370 |
| Popis: | Objective: To determine the effects of antiretroviral therapy on thymic output independent of HIV infection. Methods: Thymic output was evaluated by quantifying signal joint T-cell receptor (TCR) recombination excision circles in peripheral blood lymphocytes from HIV-negative patients undergoing prophylactic antiretroviral therapy. Additionally, effects of the HIV protease inhibitor nelfinavir were assessed in vivo on TCR-induced death of murine double-positive thymocytes. Results: Five out of seven HIV-negative patients undergoing prophylactic antiretroviral therapy exhibited a dramatic increase (1-3 log 10 ) in recent thymic emigrants containing signal joint TCR recombination excision circles while their peripheral T cell compartments remained relatively unaffected. None of the patients developed subsequent HIV infections. Interestingly, nelfinavir did not have significant effects on TCR-induced apoptosis of murine thymocytes in vivo. Conclusion: Antiretroviral therapy augments thymic output independent of HIV. Furthermore, nelfinavir does not dramatically affect TCR-induced thymocyte death in mice, thus central tolerance remains intact. |
| Databáze: | OpenAIRE |
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