Low-molecular-weight chondroitin sulfate attenuated injury by inhibiting oxidative stress in amyloid β-treated SH-SY5Y cells
| Autor: | Fengshan Wang, Qian Zhang, Zhao Na, Yan-Na Cheng |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
SH-SY5Y Apoptosis Pharmacology medicine.disease_cause Superoxide dismutase 03 medical and health sciences chemistry.chemical_compound Neuroblastoma 0302 clinical medicine Cell Line Tumor medicine Humans Chondroitin sulfate chemistry.chemical_classification Amyloid beta-Peptides biology General Neuroscience Glutathione peroxidase Chondroitin Sulfates Neurotoxicity Malondialdehyde medicine.disease Molecular Weight Oxidative Stress 030104 developmental biology Neuroprotective Agents chemistry biology.protein Reactive Oxygen Species 030217 neurology & neurosurgery Oxidative stress |
| Zdroj: | Neuroreport. 29(14) |
| ISSN: | 1473-558X |
| Popis: | The neurotoxicity of aggregated amyloid β (Aβ) has been implicated as a critical cause in the pathogenesis of Alzheimer's disease. In a previous work, we have shown that low-molecular-weight chondroitin sulfate (LMWCS), a derivative of chondroitin sulfate, protected the SH-SY5Y neuroblastoma cells from Aβ25-35-induced neurotoxicity, decreased intracellular reactive oxygen species level and inhibited the cell apoptosis. However, the underlying mechanism of the antioxidative effect of LMWCS in the SH-SY5Y cells has not been well explored. In the present study, the SH-SY5Y cells were cultured and exposed to 30 μM Aβ25-35 in the absence or presence of LMWCS (50, 100 and 200 μg/ml). Results indicate that incubation of cells with LMWCS before Aβ25-35 exposure increased superoxide dismutase, glutathione peroxidase and Na/K-ATPase activities and decreased the malondialdehyde content. In addition, LMWCS inhibited the imbalance of Bcl-2 and Bax and decreased caspase-3 and caspase-9 expressions. LMWCS antagonizes Aβ25-35-induced neurotoxicity by attenuating oxidative stress, and our results suggest that LMWCS might be used as a potential compound for Alzheimer's disease prevention. |
| Databáze: | OpenAIRE |
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