MIG--differential gene expression in mouse brain endothelial cells
| Autor: | Paola, Ghersa, Maurizio, Gelati, Jacques, Colinge, Georg, Feger, Christine, Power, Ruben, Papoian, Andrea, Salmaggi |
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| Rok vydání: | 2002 |
| Předmět: |
Chemokine
Receptors CXCR3 medicine.medical_treatment Gene Expression Biology Chemokine CXCL9 Proinflammatory cytokine Interferon-gamma Mice stomatognathic system medicine Tumor Cells Cultured Animals Oligonucleotide Array Sequence Analysis Tumor Necrosis Factor-alpha General Neuroscience Monocyte Brain Cell biology Up-Regulation Monokine Endothelial stem cell stomatognathic diseases Mononuclear cell infiltration Chemotaxis Leukocyte Drug Combinations Cytokine medicine.anatomical_structure Immunology biology.protein Intercellular Signaling Peptides and Proteins Interleukin-2 Tumor necrosis factor alpha Receptors Chemokine Endothelium Vascular Chemokines CXC Interleukin-1 |
| Zdroj: | Neuroreport. 13(1) |
| ISSN: | 0959-4965 |
| Popis: | Different diseases of the CNS are associated with blood-brain barrier (BBB) damage and mononuclear cell infiltration. In order to study genes that may play a role in endothelial cell regulation in inflammatory CNS diseases, we performed differential gene expression (DGE) analysis using a mouse brain endothelial cell line. We found that interferon-gamma (IFNgamma)-induced monokine (MIG), a chemokine that plays a role in T lymphocyte and monocyte chemoattraction, is highly expressed in the presence of inflammatory cytokines. We show that MIG, produced by brain endothelial cells in vitro, is biologically active in attractingT lymphocytes and that it is possible to interfere with this mechanism of action using anti-MIG antibodies. We suggest that blocking MIG may be beneficial in CNS inflammation. We detected constitutive expression of the MIG receptor, CXCR3, on the surface of the endothelial cells and therefore hypothesize that it plays a role in maintaining the cytokine gradient at the region of CNS inflammation. |
| Databáze: | OpenAIRE |
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