Spongiispira norvegica gen. nov., sp. nov., a marine bacterium isolated from the boreal sponge Isops phlegraei
| Autor: | Ulrich Szewzyk, Hans von Döhren, Preben Nielsen, Walter Michaelis, Ingeborg Graeber, Ines Kaesler, Rudi Lurz, Ralf Dieckmann, Thomas Pape, Martin Borchert |
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| Rok vydání: | 2008 |
| Předmět: |
DNA
Bacterial Arabinose Molecular Sequence Data Marine Biology Spirillum DNA Ribosomal Microbiology Esterase chemistry.chemical_compound Species Specificity Phylogenetics RNA Ribosomal 16S Gammaproteobacteria Botany Animals Lipase Phylogeny Ecology Evolution Behavior and Systematics Base Composition biology Norway Fatty Acids Genes rRNA Sequence Analysis DNA General Medicine biology.organism_classification 16S ribosomal RNA Bacterial Typing Techniques Porifera Phenotype chemistry biology.protein Bacteria |
| Zdroj: | INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY. 58:1815-1820 |
| ISSN: | 1466-5034 1466-5026 |
| Popis: | The bacterial strain Gp_4_7.1T, isolated from the marine sponge Isops phlegraei collected at the Sula Ridge off the Norwegian coast, was characterized. The isolate was a motile spirillum that was monopolarly and monotrichously flagellated. It was aerobic, Gram-negative, oxidase-positive and catalase-negative. Optimal growth occurred between 20 and 30 degrees C, at pH 7-8 and with a salt concentration of 2-3 % (w/v). The isolate showed a relatively restricted nutritional profile. Substrate utilization tests were only positive for arabinose. Enzyme tests were positive for esterase lipase C8, lipase C14, leucine arylamidase and naphthol-AS-BI-phosphohydrolase. The strain was not able to reduce nitrate. The major cellular fatty acids were C16:1 omega7 and C16:0. The DNA G+C content was 62.6 mol%. Phylogenetic analysis based on 16S rRNA gene sequence comparison classified the strain as a member of the order Oceanospirillales in the class Gammaproteobacteria. Strain Gp_4_7.1T formed a distinct phyletic line with less than 94 % 16S rRNA gene sequence similarity to its closest relatives with validly published names. Based on the determined data, it is proposed that the strain represents a novel species in a new genus, Spongiispira norvegica gen. nov., sp. nov.; the type strain of Spongiispira norvegica is Gp_4_7.1T (=DSM 17749T =NCIMB 14401T). |
| Databáze: | OpenAIRE |
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