Transendothelial migration induces differential migration dynamics of leukocytes in tissue matrix
| Autor: | David J. Beebe, Rouven Schoppmeyer, Martijn A. Nolte, Jaap D. van Buul, Max Blokker, Lanette Kempers, Abraham C.I. van Steen |
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| Přispěvatelé: | AII - Inflammatory diseases, ACS - Microcirculation, LaserLaB - Biophotonics and Microscopy, Biophotonics and Medical Imaging, Anatomy and neurosciences |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Neutrophils
medicine.medical_treatment Endothelial cells Inflammation Physiological hydrogel Matrix (biology) Biology Extracellular matrix Downregulation and upregulation Blood vessels medicine Leukocytes Humans Actin Tissue Chemistry Transendothelial and Transepithelial Migration Chemotaxis Cell Biology Leukocyte extravasation Cell biology Tools and Resources Migration dynamics Cytokine Tumor necrosis factor alpha Endothelium Vascular medicine.symptom Blood-vessel-on-a-chip Leukocyte transendothelial migration Vasoconstriction |
| Zdroj: | Journal of cell science, 134(21):jcs258690. Company of Biologists Ltd Journal of cell science, 134(21):jcs258690, 1-14. Company of Biologists Ltd J Cell Sci Van Steen, A C I, Kempers, L, Schoppmeyer, R, Blokker, M, Beebe, D J, Nolte, M A & Van Buul, J D 2021, ' Transendothelial migration induces differential migration dynamics of leukocytes in tissue matrix ', Journal of cell science, vol. 134, no. 21, jcs258690, pp. 1-14 . https://doi.org/10.1242/jcs.258690 Journal of Cell Science, 134(21):jcs258690. Company of Biologists Ltd van Steen, A C I, Kempers, L, Schoppmeyer, R, Blokker, M, Beebe, D J, Nolte, M A & van Buul, J D 2021, ' Transendothelial migration induces differential migration dynamics of leukocytes in tissue matrix ', Journal of Cell Science, vol. 134, no. 21, jcs258690 . https://doi.org/10.1242/jcs.258690 |
| ISSN: | 1477-9137 0021-9533 |
| DOI: | 10.1242/jcs.258690 |
| Popis: | Leukocyte extravasation into inflamed tissue is a complex process that is difficult to capture as a whole in vitro. We employed a blood-vessel-on-a-chip model in which endothelial cells were cultured in a tube-like lumen in a collagen-1 matrix. The vessels are leak-tight, creating a barrier for molecules and leukocytes. Addition of inflammatory cytokine TNF-α caused vasoconstriction, actin remodelling and upregulation of ICAM-1. Introducing leukocytes into the vessels allowed real-time visualisation of all different steps of the leukocyte transmigration cascade including migration into the extracellular matrix. Individual cell tracking over time distinguished striking differences in migratory behaviour between T-cells and neutrophils. Neutrophils cross the endothelial layer more efficiently than T-cells, but upon entering the matrix, neutrophils display high speed but low persistence, whereas T-cells migrate with low speed and rather linear migration. In conclusion, 3D imaging in real-time of leukocyte extravasation in a vessel-on-a-chip enables detailed qualitative and quantitative analysis of different stages of the full leukocyte extravasation process in a single assay.SummaryA functional hydrogel-based blood-vessel-on-a-chip model is used to study the complete leukocyte transendothelial migration process in real time. T-lymphocytes and neutrophils exhibit distinct migration dynamics in the extravascular matrix after transendothelial migration, which can be altered using a chemotactic gradient. |
| Databáze: | OpenAIRE |
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