Direct Radioimmunoassay (RIA) of salivary testosterone: correlation with free and total serum testosterone
| Autor: | S.C. Rappaport, A L Southren, Gary G. Gordon, J. Vittek, D l'Hommedieu |
|---|---|
| Rok vydání: | 1985 |
| Předmět: |
Adult
Male medicine.medical_specialty Saliva Radioimmunoassay General Biochemistry Genetics and Molecular Biology Correlation Internal medicine medicine Humans Endocrine system Testosterone General Pharmacology Toxicology and Pharmaceutics Aged biology business.industry Testosterone (patch) Salivary testosterone General Medicine Middle Aged biology.organism_classification Endocrinology Tasa Hypertension Female Menopause business Contraceptives Oral Hormone |
| Zdroj: | Life Sciences. 37:711-716 |
| ISSN: | 0024-3205 |
| DOI: | 10.1016/0024-3205(85)90540-5 |
| Popis: | Simple and sensitive direct RIA for determination of salivary testosterone was developed by using RSL NOSOLVEX TM (125 1) kit produced by Radioassay System Laboratories (Carson, California). In addition, a relationship between salivary and serum free and total testosterone concentrations was studied in randomly selected 45 healthy subjects, 5 females on oral contraceptive pills and 28 hypertensive patients on various treatment regimens. The lowest weight of testosterone detectable by our modified method was equivalent to 1 pg/ml of saliva, taking into account analytical variability. Intra- and interassay coefficients of variation were 5.09 +/- 2.7% and 8.2 +/- 5.9% respectively. Statistically significant correlations were found between salivary and serum free testosterone (r = 0.97) and salivary and serum total testosterone concentrations (r = 0.70-0.87). The exception to this was a group of hypertensive females in which no correlation (r = 0.14) between salivary and total serum testosterone was found. It is also of interest that, while salivary testosterone was significantly increased in subjects taking oral contraceptives and most of the hypertensive patients the total serum testosterone concentration was in normal range. Our findings suggest that determination of salivary testosterone is a reliable method to detect changes in the concentration of available biologically active hormone in the circulation. |
| Databáze: | OpenAIRE |
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