Using Azobenzene Incorporated DNA Aptamers to Probe Molecular Binding Interactions
| Autor: | Haipeng Liu, Joseph A. Phillips, Weihong Tan, Mingxu You, Ruowen Wang, Meghan B. O’Donoghue, Xiangling Xiong, Kwame Sefah |
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| Rok vydání: | 2011 |
| Předmět: |
Conformational change
Light Stereochemistry Aptamer Biomedical Engineering Molecular binding Pharmaceutical Science Bioengineering Article chemistry.chemical_compound Tumor Cells Cultured Humans Binding site Protein secondary structure Pharmacology Binding Sites Molecular Structure Chemistry Organic Chemistry Rational design food and beverages Stereoisomerism Aptamers Nucleotide Flow Cytometry Azobenzene Molecular Probes Biophysics Molecular probe Azo Compounds Biotechnology |
| Zdroj: | Bioconjugate Chemistry. 22:282-288 |
| ISSN: | 1520-4812 1043-1802 |
| DOI: | 10.1021/bc100402p |
| Popis: | The rational design of DNA/RNA aptamers for use as molecular probes depends on a clear understanding of their structural elements in relation to target-aptamer binding interactions. We present a simple method to create aptamer probes that can occupy two different structural states. Then, based on the difference in binding affinity between these states, target-aptamer binding interactions can be elucidated. The basis of our two-state system comes from the incorporation of azobenzene within the DNA strand. Azobenzene can be used to photo-regulate the melting of DNA-duplex structures. When incorporated into aptamers, the light-regulated conformational change of azobenzene can be used to analyze how aptamer secondary structure is involved in target binding. Azobenzene modified aptamers showed no change in target selectivity, but showed differences in binding affinity as a function of the number, position, and conformation of azobenzene modifications. Aptamer probes that can change binding affinity on demand may have future uses in targeted drug delivery and photodynamic therapy. |
| Databáze: | OpenAIRE |
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