Survey of human immunodeficiency virus (HIV)-seropositive patients with mycobacterial infection in Japan
| Autor: | Jun-ichiro Sekiguchi, Hideyuki Koga, Yayoi Otsuka, Namiko Mori, Satoshi Kimura, Takuma Shirasaka, Yoshihiro Kikuchi, Katsutoshi Saruta, Hideo Saka, Tomoko Fujino, Tadatoshi Kuratsuji, Makiko Kiyosuke, Teruo Kirikae, Yasuki Yamashita, Hideaki Nagai, Yoshito Otsuka, Atsushi Ajisawa, Emiko Toyota, Makoto Takahara, Toru Mori, Shinichi Oka, Yuka Sasaki |
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| Rok vydání: | 2005 |
| Předmět: |
Adult
Male Microbiology (medical) Tuberculosis Adolescent Mycobacterium chelonae HIV Infections Microbial Sensitivity Tests Biology Virus Microbiology Mycobacterium tuberculosis Japan HIV Seronegativity HIV Seropositivity Prevalence medicine Humans Child Aged Mycobacterium Infections Molecular epidemiology Middle Aged medicine.disease biology.organism_classification DNA Fingerprinting Health Surveys Virology Infectious Diseases Female Viral disease Restriction fragment length polymorphism Polymorphism Restriction Fragment Length Mycobacterium avium Mycobacterium |
| Zdroj: | Journal of Infection. 51:364-374 |
| ISSN: | 0163-4453 |
| DOI: | 10.1016/j.jinf.2004.12.015 |
| Popis: | Objective To assess DNA polymorphisms in mycobacterial isolates obtained from human immunodeficiency virus (HIV)-seropositive patients with tuberculosis in Japan from 1996 to 2003. Methods Restriction fragment length polymorphisms (RFLP) from Mycobacterium tuberculosis and Mycobacterium avium isolates obtained from individual seropositive patients with tuberculosis ( n =78) were analysed with the use of IS 6110 and (CGG) 5 or IS 1245 and IS 1311 , respectively, as markers. As a control, the same procedures were applied to isolates from HIV-seronegative tuberculosis patients ( n =87). Results Of 86 mycobacterial strains, M. tuberculosis , M. avium and Mycobacterium chelonae were identified in 48 (55.8%), 36 (41.9%) and 2 (2.3%) isolates, respectively. The obtained RFLP patterns of M. tuberculosis isolates from both the HIV-seropositive and -seronegative groups were variable, suggesting no obvious clustering among the isolates. Similar results were obtained in isolates of M. avium . Conclusions This is the first report on the molecular epidemiology of Mycobacterium spp. isolated from HIV-seropositive patients in Japan. The results indicate that no particular clones of M. tuberculosis or M. avium prevail in HIV-seropositive patients in Japan. Further monitoring of mycobacterial infection associated with HIV infection in Japan should be continued. |
| Databáze: | OpenAIRE |
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