Engineering a thermoregulated intein-modified xylanase into maize for consolidated lignocellulosic biomass processing
| Autor: | Benjamin N. Gray, Matthew Parker, Vladimir Samoylov, Hector Lucero, Oleg Bougri, R. Michael Raab, Mary Ross, Binzhang Shen, Elaina Hancock, Gabor Lazar, Jeremy Johnson, Nathan A. Ekborg, Taran C. Shilling, Dongcheng Zhang, Xiao Zuo, Xueguang Sun, James Apgar |
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| Rok vydání: | 2012 |
| Předmět: |
Biomedical Engineering
Biomass Lignocellulosic biomass Bioengineering Xylose complex mixtures Applied Microbiology and Biotechnology Lignin Zea mays Inteins chemistry.chemical_compound Food science Stover Endo-1 4-beta Xylanases Chemistry fungi food and beverages Plants Genetically Modified Genetic Enhancement Agronomy Cellulosic ethanol Biofuel Xylanase Molecular Medicine Intein Biotechnology Body Temperature Regulation |
| Zdroj: | Nature biotechnology. 30(11) |
| ISSN: | 1546-1696 |
| Popis: | Plant cellulosic biomass is an abundant, low-cost feedstock for producing biofuels and chemicals. Expressing cell wall-degrading (CWD) enzymes (e.g. xylanases) in plant feedstocks could reduce the amount of enzymes required for feedstock pretreatment and hydrolysis during bioprocessing to release soluble sugars. However, in planta expression of xylanases can reduce biomass yield and plant fertility. To overcome this problem, we engineered a thermostable xylanase (XynB) with a thermostable self-splicing bacterial intein to control the xylanase activity. Intein-modified XynB (iXynB) variants were selected that have10% wild-type enzymatic activity but recover60% enzymatic activity upon intein self-splicing at temperatures59 °C. Greenhouse-grown xynB maize expressing XynB has shriveled seeds and low fertility, but ixynB maize had normal seeds and fertility. Processing dried ixynB maize stover by temperature-regulated xylanase activation and hydrolysis in a cocktail of commercial CWD enzymes produced90% theoretical glucose and63% theoretical xylose yields. |
| Databáze: | OpenAIRE |
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