Production and characterization of recombinant transmembrane proteins from Mycoplasma hyopneumoniae
| Autor: | Clarice Brinck Brum, Vanessa Galli, Simone Simionatto, Charles K. Gomes, Odir Antônio Dellagostin, Andressa Fisch, Fabricio Rochedo Conceição, Silvana Beutinger Marchioro |
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| Rok vydání: | 2012 |
| Předmět: |
Male
Biology medicine.disease_cause Microbiology law.invention Mice Immune system Antigen Mycoplasma hyopneumoniae law Escherichia coli medicine Animals Gene Antigens Bacterial Vaccines Synthetic General Veterinary Reverse vaccinology Membrane Proteins General Medicine biology.organism_classification Antibodies Bacterial Virology Recombinant Proteins Transmembrane protein Immunity Humoral Recombinant DNA Female |
| Zdroj: | Veterinary Microbiology. 155:44-52 |
| ISSN: | 0378-1135 |
| DOI: | 10.1016/j.vetmic.2011.08.001 |
| Popis: | Mycoplasma hyopneumoniae is the etiological agent of swine enzootic pneumonia (EP), a chronic respiratory disease which causes significant economic losses to the swine industry worldwide. More efficient strategies for controlling this disease are necessary. In this study, we cloned17 genes coding for transmembrane proteins from M. hyopneumoniae, among which six were successfully expressed in Escherichia coli and had their immunogenic and antigenic properties evaluated. All proteins were immunogenic in mice and sera from naturally infected pigs reacted with the recombinant proteins, suggesting that they are expressed during infection. These antigens may contribute for the development of new recombinant vaccines and diagnostic tests against EP. |
| Databáze: | OpenAIRE |
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