Usefulness of matrix-assisted laser desorption ionization/time of flight mass spectrometry for the identification of Streptococcus mutans

Autor:	Clara Fernández Álvarez, Yolanda Torres Corral, Ysabel Santos, Antonio Aguilera Guirao, Rafael Seoane Prado, Nancy Saltos Rosero
Rok vydání:	2020
Předmět:	Serotype 0303 health sciences Saliva biology 030306 microbiology Lasers Reproducibility of Results Streptococcus Matrix assisted laser desorption ionization time of flight General Medicine biology.organism_classification Mass spectrometry Applied Microbiology and Biotechnology Streptococcus mutans Microbiology 03 medical and health sciences Matrix-assisted laser desorption/ionization Species level Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization Potential biomarkers 030304 developmental biology Biotechnology
Zdroj:	Applied Microbiology and Biotechnology. 104:10601-10612
ISSN:	1432-0614 0175-7598
DOI:	10.1007/s00253-020-10980-7
Popis:	This study evaluated the reliability of MALDI-TOF MS coupled with statistical tools for the identification of Streptococcus mutans in comparison with PCR-based techniques. Bacterial isolates were identified and serotyped by conventional PCR, using S. mutans species and serotype-specific primers. For bacterial identification, mass spectra data from S. mutans and other streptococci were compared with Biotyper V 3.1 database and the mass peak lists were examined by cluster and principal component (PCA) analysis. Identification of potential biomarkers was performed using UniProtKB/Swiss-Prot and UniProtKB/TrEMBL databases and BLAST tool of the NCBI database. PCR identified 100% of the isolates as S. mutans. S. mutans strains were typed as serotypes c (85.6%), e (8.6%), k (4.8%), and f (0.9%). Although only the 70% of the strains tested were identified at species level by the Biotyper database, PCA and cluster analysis of mass peaks allowed the identification of 100% S. mutans isolates and its differentiation from the other oral and non-oral streptococci. One mass peak at m/z value of 9572.73 was identified as species-specific biomarker for S. mutans. No biomarkers were identified for S. mutans serotypes. KEY POINTS: • MALDI-TOF MS coupled with statistical tools for the identification of S. mutans. • Detection of species identifying biomarkers by MALDI-TOF MS. • PCR identification and serotyping of S. mutans from saliva samples.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b6dfe627c76fcefa5c8f377c76f0033c https://doi.org/10.1007/s00253-020-10980-7 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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