Preferential accumulation within tumors and in vivo imaging by functionalized luminescent dendrimer lanthanide complexes

Autor: Marco A. Alcala, Hyounsoo Uh, Yong J. Lee, Ruth A. Modzelewski, Shu Ying Kwan, Kristy A. Gogick, David L. Bartlett, Stéphane Petoud, Adam R. Meier, Matthias Bischof, Chad M. Shade, Timothy G. Strein, Charles K. Brown, Zachary P. Thompson
Přispěvatelé: School Medicine, University of Pittsburgh (PITT), Pennsylvania Commonwealth System of Higher Education (PCSHE)-Pennsylvania Commonwealth System of Higher Education (PCSHE), Carnegie Mellon University [Pittsburgh] (CMU), Department of Chemistry, Centre de biophysique moléculaire (CBM), Université d'Orléans (UO)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)
Rok vydání: 2011
Předmět:
Male
Pathology
MESH: Europium
Luminescence
Time Factors
MESH: Electrophoresis
01 natural sciences
MESH: Infusions
Intra-Arterial
MESH: Liver Neoplasms
MESH: Animals
MESH: Luminescence
Liver Neoplasms
Fluorescence
Imaging agent
3. Good health
Mechanics of Materials
Colorectal Neoplasms
Europium
MESH: Spectrometry
Fluorescence
Preclinical imaging
Diagnostic Imaging
Electrophoresis
Dendrimers
medicine.medical_specialty
MESH: Cell Line
Tumor
Materials science
MESH: Rats
Biophysics
chemistry.chemical_element
Bioengineering
In Vitro Techniques
010402 general chemistry
Article
Biomaterials
Hepatic arterial infusion
Cell Line
Tumor
Dendrimer
medicine
Animals
Infusions
Intra-Arterial
[SDV.BBM]Life Sciences [q-bio]/Biochemistry
Molecular Biology
MESH: Dendrimers
MESH: Diagnostic Imaging
010405 organic chemistry
MESH: Time Factors
Photobleaching
MESH: Male
Rats
0104 chemical sciences
Spectrometry
Fluorescence
chemistry
Ceramics and Composites
MESH: Colorectal Neoplasms
Zdroj: Biomaterials
Biomaterials, Elsevier, 2011, 32 (35), pp.9343-52. ⟨10.1016/j.biomaterials.2011.07.076⟩
ISSN: 0142-9612
Popis: International audience; We have created a dendrimer complex suitable for preferential accumulation within liver tumors and luminescence imaging by substituting thirty-two naphthalimide fluorophores on the surface of the dendrimer and incorporating eight europium cations within the branches. We demonstrate the utility and performance of this luminescent dendrimer complex to detect hepatic tumors generated via direct subcapsular implantation or via splenic injections of colorectal cancer cells (CC531) into WAG/RijHsd rats. Luminescence imaging of the tumors after injection of the dendrimer complex via hepatic arterial infusion revealed that the dendrimer complex can preferentially accumulate within liver tumors. Further investigation indicated that dendrimer luminescence in hepatic tumors persisted in vivo. Due to the incorporation of lanthanide cations, this luminescence agent presents a strong resistance against photobleaching. These studies show the dendrimer complex has great potential to serve as an innovative accumulation and imaging agent for the detection of metastatic tumors in our rat hepatic model.
Databáze: OpenAIRE
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