Weirdo19ES is a novel singleton mycobacteriophage that selects for glycolipid deficient phage-resistant M. smegmatis mutants

Autor: Sabrina Emilse Tasselli, Cristian Alejandro Suárez, Jorgelina Judith Franceschelli, Héctor R. Morbidoni
Jazyk: angličtina
Rok vydání: 2020
Předmět:
Lysin
Pathology and Laboratory Medicine
Siphoviridae
purl.org/becyt/ford/1 [https]
Database and Informatics Methods
Cell Wall
Medicine and Health Sciences
Cluster Analysis
Bacteriophages
Codon Usage
Phylogeny
Genetics
0303 health sciences
Base Composition
Comparative Genomic Hybridization
Multidisciplinary
biology
Mycobacteriophages
Mycobacterium smegmatis
BIOINFORMATICS
Genomics
Genomic Databases
Bacterial Pathogens
Actinobacteria
Phenotype
Medical Microbiology
Viruses
Medicine
Pathogens
Research Article
MYCOBACTERIOPHAGE
food.ingredient
Mycobacteriophage
Science
Genome
Viral
Gordonia
Research and Analysis Methods
Microbiology
03 medical and health sciences
food
purl.org/becyt/ford/1.6 [https]
Gene Prediction
Molecular Biology Techniques
Gene
Microbial Pathogens
Molecular Biology
030304 developmental biology
Bacteria
030306 microbiology
Organisms
Biology and Life Sciences
Computational Biology
Mycobacteria
Comparative Genomics
biology.organism_classification
Genome Analysis
Biological Databases
MUTANTS
SINGLETON
Glycolipids
Mycobacterium Tuberculosis
Mycobacterium
Cloning
Zdroj: PLoS ONE
CONICET Digital (CONICET)
Consejo Nacional de Investigaciones Científicas y Técnicas
instacron:CONICET
PLoS ONE, Vol 15, Iss 5, p e0231881 (2020)
ISSN: 1932-6203
Popis: The sequencing and bioinformatics analysis of bacteriophages infecting mycobacteria has yielded a large amount of information on their evolution, including that on their environmental propagation on other genera such as Gordonia, closely related to Mycobacterium. However, little is known on mycobacteriophages cell biology such as the nature of their receptor (s) or their replication cycle. As part of our on-going screening for novel mycobacteriophages, we herein report the isolation and genome bioinformatics analysis of Weirdo19ES, a singleton Siphoviridae temperate mycobacteriophage with a 70.19% GC content. Nucleotide and protein sequence comparison to actinobacteriophage databases revealed that Weirdo19ES shows low homology to Gordonia phage Ruthy and mycobacteriophages falling in clusters Q and G and to singleton DS6A.Weirdo19ES also displays uncommon features such as a very short Lysin A gene (with only one enzymatic domain) and two putative HNH endonucleases. Mycobacterium smegmatis mutants resistant to Weirdo19ES are cross- resistant to I3. In agreement with that phenotype, analysis of cell envelope of those mutants showed that Weirdo19ES shares receptors with the transducing mycobacteriophage I3.This singleton mycobacteriophage adds up to the uncommonness of local mycobacteriophages previously isolated by our group and helps understanding the nature of mycobacteriophage receptors. Fil: Suárez, Cristian Alejandro. Universidad Nacional de Rosario. Facultad de Ciencias Médicas. Laboratorio de Microbiología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina Fil: Franceschelli, Jorgelina Judith. Universidad Nacional de Rosario. Facultad de Ciencias Médicas. Laboratorio de Microbiología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina Fil: Tasselli, Sabrina Emilse. Universidad Nacional de Rosario. Facultad de Ciencias Médicas. Laboratorio de Microbiología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina Fil: Morbidoni, Héctor Ricardo. Universidad Nacional de Rosario. Facultad de Ciencias Médicas. Laboratorio de Microbiología Molecular; Argentina
Databáze: OpenAIRE
Externí odkaz:
Nepřihlášeným uživatelům se plný text nezobrazuje