Diaminobenzidine as a Myelin Stain in Semithin Plastic Sections
| Autor: | S. K. Krueger, M. M. Frederick, R. K. Johnson, D. E. Phillips |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
Histology
Myelinated nerve fiber 3 3'-Diaminobenzidine Stain law.invention Rats Sprague-Dawley chemistry.chemical_compound law Polymer chemistry Microtome Animals Tissue Embedding Coloring Agents Myelin Sheath Sodium ethoxide Staining and Labeling Epoxy Resins Microtomy General Medicine Rats Medical Laboratory Technology Spinal Cord chemistry Glutaraldehyde Electron microscope Nuclear chemistry |
| Zdroj: | Biotechnic & Histochemistry. 74:105-109 |
| ISSN: | 1473-7760 1052-0295 |
| Popis: | A diaminobenzidine (DAB) stain for myelin in glutaraldehyde fixed, osmicated, semithin epoxy sections is described. One or 1.5 microm sections, dried onto slides, are first etched with a 1:2 dilution of saturated sodium ethoxide:absolute ethanol, then incubated in 0.05% aqueous DAB with 0.01% hydrogen peroxide. DAB specifically stains osmium fixed myelinated nerve fibers. This permits high resolution light microscopic study of myelinated nerve fibers in semithin sections of tissues that also can be studied by electron microscopy. |
| Databáze: | OpenAIRE |
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