Validated High Performance Liquid Chromatographic Method for Simultaneous Determination of Phenytoin, Phenobarbital and Carbamazepine in Human Serum
| Autor: | K. Rajnarayana, Devarakonda R. Krishna, Jenugu Vidya Sagar, Perumandla Kishore, Mada S. Reddy |
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| Rok vydání: | 2011 |
| Předmět: |
medicine.medical_treatment
Ethyl acetate High-performance liquid chromatography chemistry.chemical_compound Acetic acid Column chromatography Drug Discovery medicine Humans Chromatography High Pressure Liquid Epilepsy Chromatography medicine.diagnostic_test Chemistry Reproducibility of Results Carbamazepine Reference Standards Anticonvulsant Therapeutic drug monitoring Phenobarbital Phenytoin Anticonvulsants Indicators and Reagents Spectrophotometry Ultraviolet Quantitative analysis (chemistry) medicine.drug |
| Zdroj: | Arzneimittelforschung. 53:763-768 |
| ISSN: | 1616-7066 0004-4172 |
| DOI: | 10.1055/s-0031-1299826 |
| Popis: | A high performance liquid chromatographic (HPLC) method for the simultaneous determination of phenytoin (CAS 57-41-0), phenobarbital (CAS 50-06-6, phenobarbitone) and carbamazepine (CAS 298-46-4) is described. The serum was extracted with ethyl acetate, the dried extract was reconstituted in mobile phase and the aliquot was injected. The eluent drugs were detected at 230 nm. The mobile phase consisting of methanol: water: glacial acetic acid mixture (67:33:1, v/v/v) was used at a flow rate of 1 ml/min on C-18 column. The absolute recovery was above 96% of all the three drugs over a concentration range of 0.5-50.0 micrograms/ml. The inter-day and intra-day precision relative standard deviation (RSD) ranged from 0.79-5.13% and 0.11-6.81%, respectively. The method is simple, rapid, accurate and sensitive and is presently used for therapeutic drug monitoring in epileptic patients. The results obtained with this method showed very good clinical correlation. |
| Databáze: | OpenAIRE |
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