Lipid anchoring and electrostatic interactions target NOT-LIKE-DAD to pollen endo-plasma membrane
| Autor: | Peter M. Rogowsky, Jean-Pierre Martinant, Nathanaël M. A. Jacquier, Veronica La Padula, Claire Lionnet, Laurine M. Gilles, Andrea R M Calhau, Thomas Widiez |
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| Přispěvatelé: | Reproduction et développement des plantes (RDP), École normale supérieure - Lyon (ENS Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon, Limagrain Europe, ANR-19-CE20-0012,NOT-LIKE-DAD,Gynogenèse in vivo chez le maïs : nouvelles connaissances sur la double fécondation chez les plantes(2019), École normale supérieure de Lyon (ENS de Lyon)-Université Claude Bernard Lyon 1 (UCBL) |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
0301 basic medicine Somatic cell Static Electricity [SDV.BC]Life Sciences [q-bio]/Cellular Biology [SDV.BC.BC]Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC] Phospholipase Biology Zea mays 01 natural sciences Phospholipases A [SDV.BDLR.RS]Life Sciences [q-bio]/Reproductive Biology/Sexual reproduction 03 medical and health sciences medicine [SDV.BV]Life Sciences [q-bio]/Vegetal Biology [SDV.BDD]Life Sciences [q-bio]/Development Biology Cell Membrane food and beverages [SDV.BDLR]Life Sciences [q-bio]/Reproductive Biology [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry Molecular Biology/Molecular biology Cell Biology Lipids Sperm Cell biology Sexual reproduction Cytosol [SDV.BV.AP]Life Sciences [q-bio]/Vegetal Biology/Plant breeding 030104 developmental biology Membrane medicine.anatomical_structure Pollen Gamete Pollen tube 010606 plant biology & botany |
| Zdroj: | Journal of Cell Biology Journal of Cell Biology, Rockefeller University Press, 2021, 220 (10), ⟨10.1083/jcb.202010077⟩ Journal of Cell Biology, 2021, 220 (10), ⟨10.1083/jcb.202010077⟩ |
| ISSN: | 0021-9525 1540-8140 |
| DOI: | 10.1083/jcb.202010077⟩ |
| Popis: | International audience; Phospholipases cleave phospholipids, major membrane constituents. They are thus essential for many developmental processes, including male gamete development. In flowering plants, mutation of phospholipase NOT-LIKE-DAD (NLD, also known as MTL or ZmPLA1) leads to peculiar defects in sexual reproduction, notably the induction of maternal haploid embryos. Contrary to previous reports, NLD does not localize to cytosol and plasma membrane of sperm cells but to the pollen endo-plasma membrane (endo-PM), a specific membrane derived from the PM of the pollen vegetative cell that encircles the two sperm cells. After pollen tube burst, NLD localizes at the apical region of the egg apparatus. Pharmacological approaches coupled with targeted mutagenesis revealed that lipid anchoring together with electrostatic interactions are involved in the attachment of NLD to this atypical endo-PM. Membrane surface-charge and lipid biosensors indicated that phosphatidylinositol-4,5-bisphosphate is enriched in the endo-PM, uncovering a unique example of how membrane electrostatic properties can define a specific polar domain (i.e., endo-PM), which is critical for plant reproduction and gamete formation. |
| Databáze: | OpenAIRE |
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