Parallel substrate feeding and pH-control in shaking-flasks
| Autor: | Dirk Weuster-Botz, Matthias Arnold, Jutta Altenbach-Rehm |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Syringe driver
Environmental Engineering Chromatography Chemistry Ph control Biomedical Engineering Continuous stirred-tank reactor Substrate (chemistry) Bioengineering Control engineering equipment and supplies complex mixtures Distribution system Coli strain Laboratory flask Batch operation Biotechnology |
| Zdroj: | Biochemical Engineering Journal. 7:163-170 |
| ISSN: | 1369-703X |
| DOI: | 10.1016/s1369-703x(00)00117-0 |
| Popis: | An intermittent feeding system for shaking-flasks was developed to close the gap between batch operated shaking-flasks and fed-batch operated as well as pH-controlled stirred tank reactors. A precise syringe pump was connected via a substrate distribution system to individual 2/2-way miniature valves, one for each of up to 16 shaking-flask. The shaking-flasks were equipped with pH-probes. A process computer controls the intermittent feeding of substrates by tracking predefined individual feeding profiles as well as the base (or acid) addition for individual pH-control of the shaking-flasks. Higher concentrations of aerobic cells with higher cellular activities were achieved in fed-batch operated and pH-controlled shaking-flasks as compared to the conventional batch operation. Physiological effects of an intermittent feeding were studied in a stirred tank reactor with a recombinant E. coli strain, which expressed the GDP-mannose-pyrophosphorylase enzyme under the control of the lac-promoter. |
| Databáze: | OpenAIRE |
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