Intrinsic and extrinsic regulation of the differentiation of skin, corneal and esophageal epithelial cells
| Autor: | Alda Vidrich, Thomas I. Doran, Tung-Tien Sun |
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| Rok vydání: | 1980 |
| Předmět: |
Cell type
Transplantation Heterologous Mice Nude Stratified squamous epithelium Biology General Biochemistry Genetics and Molecular Biology Cornea Corneal Transplantation Mice Esophagus In vivo Keratin medicine Animals Cells Cultured chemistry.chemical_classification Mesenchymal stem cell Cell Differentiation Epithelial Cells Phenotype Epithelium Trypsinization Cell biology medicine.anatomical_structure chemistry Epidermal Cells Immunology Keratins Female Rabbits Epidermis |
| Zdroj: | Cell. 22(1 Pt 1) |
| ISSN: | 0092-8674 |
| Popis: | Basal cells of the stratified squamous epithelia of rabbit skin, cornea and esophagus appear morphologically similar. However, the histological features of their subsequent differentiation are different, and the three epithelia are characterized by distinctive keratin proteins. To analyze the relative importance of intrinsic versus extrinsic factors in regulating the differentiation of these epithelia, we compared their behavior under identical in vitro and in vivo conditions. When cultured in the presence of 3T3 feeder cells, keratinocytes from all three epithelia formed differentiating colonies. Although in culture the three cell types approached a common phenotype, they remained distinguishable morphologically and, in some cases, biochemically. When these cultured epithelial cells were trypsinized, suspended in medium and injected subcutaneously into athymic (nude) mice, each of the three cell types generated a characteristic cyst consisting of stratified squamous epithelium. Cultured skin, corneal and esophageal keratinocytes formed epithelia which were keratinized, nonkeratinized and parakeratinized, respectively. In addition, the injected skin and esophageal epithelial cells reacquired their distinctive in vivo keratin patterns. These data suggest that the three epithelia are not equipotential. Furthermore, since the distinctive in vivo phenotype of each epithelium was expressed when the cells were transplanted to the same subcutaneous site, the expression of these differences does not depend on specific mesenchymal instruction but on permissive factors not present in the culture system. Thus under in vivo conditions intrinsic divergence must play a predominant role in determining the characteristic phenotypes of the three epithelia. On the other hand, the finding that the morphological and biochemical differentiation of a given epithelium can be reversibly modulated by the external environment demonstrates that extrinsic factors may, under certain conditions, also play a role in regulating epithelial differentiation. |
| Databáze: | OpenAIRE |
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