Determination of N-methyl-d-aspartate in tissues of bivalves by high-performance liquid chromatography
| Autor: | Yoshio Kera, Kimihiko Shibata, Natsumi Todoroki, Ryo-hei Yamada, Takahiro Yamada |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
D aspartate
Fluorenes Oxidase test N-Methylaspartate Chromatography Chemistry Diastereomer Stereoisomerism General Chemistry High-performance liquid chromatography chemistry.chemical_compound Spectrometry Fluorescence Mollusca Animals NMDA receptor Indicators and Reagents Ethyl chloroformate Derivatization Chromatography High Pressure Liquid Derivative (chemistry) |
| Zdroj: | Journal of Chromatography B: Biomedical Sciences and Applications. 728:41-47 |
| ISSN: | 0378-4347 |
| DOI: | 10.1016/s0378-4347(99)00089-4 |
| Popis: | The natural occurrence of N-methyl-D-aspartate (NMDA) is limited to the foot muscle of Scapharca broughtonii; it is a well known compound for its neuroexitatory activity. This paper describes a high-performance liquid chromatographic (HPLC) method for the determination of NMDA in biological extracts. The method involves removal of neutral and basic substances by anion-exchange chromatography and removal of acidic primary amino acids by treatment with o-phthalaldehyde before derivatization with (+)-1-(9-fluorenyl)ethyl chloroformate, followed by HPLC with isocratic elution with a selected mobile phase that separates the two diastereomers formed. The identity of the detected NMDA has been confirmed by a procedure using (-)-1-(9-fluorenyl)ethyl chloroformate as a derivatizing agent. The identification has been further supported by the disappearance of the peak of the NMDA derivative by pretreatment of the sample with D-aspartate oxidase. Application of the method has shown the presence of NMDA in several tissues of S. broughtonii and Scapharca subcrenata. |
| Databáze: | OpenAIRE |
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