CDK-Regulated Phase Separation Seeded by Histone Genes Ensures Precise Growth and Function of Histone Locus Bodies
| Autor: | Victoria E. Deneke, Stefano Di Talia, William F. Marzluff, Marco Tarzia, James P. Kemp, Robert J. Duronio, Woonyung Hur |
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| Rok vydání: | 2020 |
| Předmět: |
Transcriptional Activation
Scaffold protein Embryonic Development Cell Cycle Proteins Article General Biochemistry Genetics and Molecular Biology Histones 03 medical and health sciences 0302 clinical medicine Transcription (biology) Cyclin-dependent kinase Gene expression Animals Drosophila Proteins RNA Messenger Histone locus body Molecular Biology 030304 developmental biology Cell Nucleus Regulation of gene expression 0303 health sciences biology Cell Cycle Cyclin-dependent kinase 2 Cell Biology Cell biology Drosophila melanogaster Histone biology.protein Drosophila 030217 neurology & neurosurgery Developmental Biology |
| Zdroj: | Dev Cell |
| ISSN: | 1534-5807 |
| DOI: | 10.1016/j.devcel.2020.06.003 |
| Popis: | Many membrane-less organelles form through liquid-liquid phase separation, but how their size is controlled and whether size is linked to function remain poorly understood. The Histone Locus Body (HLB) is an evolutionarily conserved nuclear body that regulates the transcription and processing of histone mRNAs. Here, we show that Drosophila HLBs form through phase separation. During embryogenesis, the size of HLBs is controlled in a precise and dynamic manner that is dependent on the cell cycle and zygotic histone gene activation. Control of HLB growth is achieved by a mechanism integrating nascent mRNAs at the histone locus, which facilitates phase separation, and the nuclear concentration of the scaffold protein multi-sex combs (Mxc), which is controlled by the activity of cyclin-dependent kinases. Reduced Cdk2 activity results in smaller HLBs and the appearance of nascent, misprocessed histone mRNAs. Thus, our experiments identify a mechanism linking nuclear body growth and size with gene expression. |
| Databáze: | OpenAIRE |
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