Silk fibroin films stabilizes and releases bioactive insulin for the treatment of corneal wounds
| Autor: | Osvaldo de Freitas, Karina Dias, Camila Nunes Lemos, Rondinelli Donizetti Herculano, Renata Fonseca Vianna Lopez, Camila Cubayachi, Francieli Pereira |
|---|---|
| Přispěvatelé: | Universidade de São Paulo (USP), Universidade Estadual Paulista (Unesp) |
| Rok vydání: | 2019 |
| Předmět: |
Circular dichroism
Polymers and Plastics Drug delivery system Silk fibroin General Physics and Astronomy Fibroin 02 engineering and technology 010402 general chemistry 01 natural sciences Ocular delivery In vivo Corneal wound Materials Chemistry Native state medicine Insulin Film Corneal epithelium Chemistry CICATRIZAÇÃO Organic Chemistry Biological activity 021001 nanoscience & nanotechnology 0104 chemical sciences medicine.anatomical_structure Biophysics Swelling medicine.symptom 0210 nano-technology Wound healing |
| Zdroj: | Web of Science Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP |
| ISSN: | 0014-3057 |
| Popis: | Made available in DSpace on 2019-10-06T08:11:58Z (GMT). No. of bitstreams: 0 Previous issue date: 2019-09-01 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Insulin (INS) is a protein that plays a crucial role in many cellular functions, including wound healing processes. However, its topical ocular administration is a challenge, mainly because of INS degradation and low retention time, which can significantly reduce its bioavailability. The objective of this work was to develop films based on silk fibroin (SF), a natural polymer with anti-inflammatory properties, containing INS aimed at the sustained release of a bioactive INS in the eye for the treatment of corneal wounds. SF films containing 100 IU/cm(2) INS and glycerin as plasticizer were prepared by casting. They were homogeneous, transparent, permeable to water vapor, with low swelling index and high mechanical resistance. Fourier transformed infrared spectroscopy and differential scanning calorimetry analyses suggested the beta-sheet/Silk II conformation of SF and the occurrence of non-covalent interactions between INS and SF. Scanning electron microscopy suggested that the INS was embedded inside the film in its native globular form. In fact, the INS released from the film maintained its native conformation, as observed by circular dichroism, in addition to conserving its biological activity in vivo, reducing the blood glycemia of Wistar rats. Sustained release, of approximately 1 IU/cm(2)/h(1/2) of INS per hour, following a burst release, suggests that the film could be effective in the recovery of injured corneal epithelium, avoiding the need for the various daily administrations. Therefore, SF films demonstrated potential to transport and release INS in a sustained manner, a promising strategy for the treatment of corneal wounds. Univ Sao Paulo, Sch Pharmaceut Sci Ribeirao Preto, Ribeirao Preto, SP, Brazil Sao Paulo State Univ, Sch Pharmaceut Sci, Araraquara, SP, Brazil Sao Paulo State Univ, Sch Pharmaceut Sci, Araraquara, SP, Brazil FAPESP: 2014/19018-0 FAPESP: 2014/22451-7 CAPES: 2014/19018-0 CAPES: 2014/22451-7 CNPq: 162175/2014-0 |
| Databáze: | OpenAIRE |
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