Reactivity of cross-reacting monoclonal antibodies with canine leukocytes, platelets and erythrocytes
| Autor: | Martin Faldyna, Rea-Min Chu, Hans-Joachim Schuberth, Gintare Kucinskiene |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
Blood Platelets
Erythrocytes medicine.drug_class Immunology Cross Reactions medicine.disease_cause Monoclonal antibody Cross-reactivity Epitope Flow cytometry Dogs Antigens CD medicine Leukocytes Animals Humans Platelet General Veterinary biology medicine.diagnostic_test CD22 CD44 Antibodies Monoclonal Molecular biology biology.protein CD81 |
| Zdroj: | Veterinary immunology and immunopathology. 119(1-2) |
| ISSN: | 0165-2427 |
| Popis: | A panel of 380 commercially available monoclonal antibodies (mAbs) against human CD molecules from various sources was tested during the 8th Human Leukocyte Differentiation Antigen Workshop (HLDA8) for cross-reactivity on canine peripheral blood leukocytes by flow cytometry. In addition, all mAbs were used to label a 50:50 mixture of platelets and erythrocytes of the same dogs. This testing resulted in 51 cross-reacting mAbs. mAbs with specificity for CD9, CD29, CD42a, CD61, and CD41/CD61 showed cross-reactivity with canine platelets in a non-polymorphic and one mAb with the erythrocyte antigen CD235a in a polymorphic reaction pattern. Canine leukocyte-reactive mAbs included those with specificity for CD11a, CD11b, CD14, CD18, CD21, CD22, CD47, CD49d, CD49e, CD56, CD62L, CD91, CD94, and CD172a. In addition, several mAbs resulted in a staining pattern of canine cells which suggest that the canine epitope equivalents have an alternate expression pattern from that expected for humans (CD1a, CD35, CD44, CD45, CD75s, CD81). In summary, this study confirmed the reactivity of previously described cross-reactive mAbs with canine cells and resulted in the characterization of mAbs recognizing so far undetectable canine CD molecules. |
| Databáze: | OpenAIRE |
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