Insulin resistance of protein anabolism accompanies that of glucose metabolism in lean, glucose-tolerant offspring of persons with type 2 diabetes
| Autor: | Errol B. Marliss, Marie Lamarche, Stéphanie Chevalier, Vikram Chandurkar, Sergio A. Burgos, Michael A. Tsoukas, José A. Morais |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty endocrine system diseases Anabolism Endocrinology Diabetes and Metabolism Glucose uptake Protein metabolism Type 2 diabetes Carbohydrate metabolism Offspring 03 medical and health sciences chemistry.chemical_compound Insulin resistance Glucose Metabolism Diabetes mellitus Internal medicine medicine Insulin Resistance In Vivo Protein Metabolism business.industry nutritional and metabolic diseases medicine.disease Protein catabolism Metabolism 030104 developmental biology Endocrinology chemistry business |
| Zdroj: | BMJ Open Diabetes Research & Care |
| ISSN: | 2052-4897 |
| Popis: | Objective To test whether protein anabolic resistance is an early defect in type 2 diabetes (T2D). Research design and methods Seven lean, normoglycemic T2D offspring (T2D-O) and eight matched participants without family history (controls; C) underwent a 3-hour hyperinsulinemic (40 mU/m2/min), euglycemic (5.5 mmol/L) and isoaminoacidemic clamp. Whole-body glucose and protein kinetics were measured with d-[3–3H]glucose and l-[l-13C]leucine, respectively. Plasma amino acids were measured by liquid chromatography-tandem mass spectrometry. Results Fasting glycemia and glucose kinetic variables did not differ between groups. Clamp decreases in glucose rate of appearance were not different, but rate of disappearance increased 29% less in T2D-O, to a significantly lower rate. Fasting leucine was higher in T2D-O, but kinetics did not differ. Clamp increases in leucine oxidation and decreases in endogenous rate of appearance (protein breakdown) were equal, but in T2D-O, non-oxidative rate of disappearance (protein synthesis) did not increase and net balance (synthesis—breakdown) did not become positive as in C. Conclusions Resistance of whole-body protein anabolism (synthesis and net balance) accompanies resistance of glucose uptake in T2D-O. Mechanisms responsible, possible roles in the increased risk of developing diabetes, and its potential impact on long-term protein balance require definition. |
| Databáze: | OpenAIRE |
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