Investigation of cellular responses upon interaction with silver nanoparticles
| Autor: | Seong Soo An, Ramesh Subbiah, Seong Beom Jeon, Kwideok Park, Kyu-Sik Yun, Sang Jung Ahn |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Stromal cell
Materials science Silver nanoindentation Cell Survival Cell Biophysics Pharmaceutical Science Metal Nanoparticles Bioengineering Nanotechnology Silver nanoparticle biomechanics Cell Line Biomaterials Mice International Journal of Nanomedicine Drug Discovery medicine Animals Humans Viability assay Cytotoxicity Original Research roughness Bacteria Organic Chemistry Force spectroscopy General Medicine Anti-Bacterial Agents AFM biomarker cytotoxicity medicine.anatomical_structure Cell culture NIH 3T3 Cells Antibacterial activity |
| Zdroj: | International Journal of Nanomedicine INTERNATIONAL JOURNAL OF NANOMEDICINE(10) |
| ISSN: | 1178-2013 1176-9114 |
| Popis: | Ramesh Subbiah,1,2 Seong Beom Jeon,3,4 Kwideok Park,1,2 Sang Jung Ahn,4,5 Kyusik Yun3 1Center for Biomaterials, Korea Institute of Science and Technology, Seoul, 2Department of Biomedical Engineering, Korea University of Science and Technology, Daejon, 3Department of Bionanotechnology, Gachon University, Gyeonggi-do, 4Centre for Advanced Instrumentation, Korea Research Institute of Standard and Science, 5Major of Nano Science, Korea University of Science and Technology, Daejeon, Republic of Korea Abstract: In order for nanoparticles (NPs) to be applied in the biomedical field, a thorough investigation of their interactions with biological systems is required. Although this is a growing area of research, there is a paucity of comprehensive data in cell-based studies. To address this, we analyzed the physicomechanical responses of human alveolar epithelial cells (A549), mouse fibroblasts (NIH3T3), and human bone marrow stromal cells (HS-5), following their interaction with silver nanoparticles (AgNPs). When compared with kanamycin, AgNPs exhibited moderate antibacterial activity. Cell viability ranged from ≤80% at a high AgNPs dose (40 µg/mL) to >95% at a low dose (10 µg/mL). We also used atomic force microscopy-coupled force spectroscopy to evaluate the biophysical and biomechanical properties of cells. This revealed that AgNPs treatment increased the surface roughness (P |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|