Interpretation of anomalously long crosslinks in ribosome crosslinking reveals the ribosome interaction in stationary phase E. coli

Autor: Santosh A. Misal, Bingqing Zhao, James P. Reilly
Rok vydání: 2022
Předmět:
Zdroj: RSC Chemical Biology. 3:886-894
ISSN: 2633-0679
Popis: Crosslinking mass spectrometry (XL-MS) of bacterial ribosomes revealed the dynamic intra- and intermolecular interactions within the ribosome structure. It has been also extended to capture the interactions of ribosome binding proteins during translation. Generally, XL-MS often identified the crosslinks within a cross-linkable distance (40 Å) using amine-reactive crosslinkers. The crosslinks larger than cross-linkable distance (40 Å) are always difficult to interpret and remain unnoticed. Here, we focused on stationary phase bacterial ribosome crosslinking that yields ultra-long crosslinks in an
Databáze: OpenAIRE