Gene/protein expression of CAPN1/2-CAST system members is associated with ERK1/2 kinases activity as well as progression and clinical outcome in human laryngeal cancer
| Autor: | Marian Danilewicz, Olga Stasikowska-Kanicka, Katarzyna Kolary, Jakub Miazga, Ewa Forma, Magdalena Bryś, Michał Skóra, Anna Krześlak, Paweł Jóźwiak, Katarzyna Starska, Iwona Lewy-Trenda |
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| Rok vydání: | 2016 |
| Předmět: |
Male
0301 basic medicine Oncology medicine.medical_specialty Pathology Kaplan-Meier Estimate Biology 03 medical and health sciences 0302 clinical medicine Western blot Risk Factors Internal medicine medicine Humans RNA Messenger Laryngeal Neoplasms Pathological Aged Calpastatin Aged 80 and over Mitogen-Activated Protein Kinase 1 Messenger RNA Mitogen-Activated Protein Kinase 3 medicine.diagnostic_test Calpain Kinase Calcium-Binding Proteins Cancer General Medicine Middle Aged Prognosis medicine.disease Immunohistochemistry Tumor Burden Phenotype 030104 developmental biology Laryngeal Mucosa 030220 oncology & carcinogenesis Disease Progression biology.protein Female Neoplasm Grading Neoplasm Recurrence Local |
| Zdroj: | Tumor Biology. 37:13185-13203 |
| ISSN: | 1423-0380 1010-4283 |
| Popis: | Recent evidence indicates the involvement of calpains (CAPNs), a family of cysteine proteases, in cancer development and progression, as well as the insufficient response to cancer therapies. The contribution of CAPNs and regulatory calpastatin (CAST) and ERK1/2 kinases to aggressiveness, disease course, and outcome in laryngeal cancer remains elusive. This study was aimed to evaluate the CAPN1/2-CAST-ERK1/2 enzyme system mRNA/protein level and to investigate whether they can promote the dynamic of tumor growth and prognosis. The mRNA expression of marker genes was determined in 106 laryngeal cancer (SCLC) cases and 73 non-cancerous adjacent mucosa (NCLM) controls using quantitative real-time PCR. The level of corresponding proteins was analyzed by Western Blot. SLUG expression, as indicator of pathological advancement was determined using IHC staining. Significant increases of CAPN1/2-CAST-ERK1/2 levels of mRNA/protein were noted in SCLC compared to NCLM (p |
| Databáze: | OpenAIRE |
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