Antibacterial and Antifungal Activities of Vasostatin-1, the N-terminal Fragment of Chromogranin A
| Autor: | Karine Lugardon, Yannick Goumon, Dominique Aunis, Angelo Corti, Marie-Hélène Metz-Boutigue, Philippe Bulet, Roselyne Raffner, Agnès F. Delmas |
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| Přispěvatelé: | Biologie de la communication cellulaire, IFR 31 Louis Bugnard (IFR 31), Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-CHU Toulouse [Toulouse]-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-CHU Toulouse [Toulouse]-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM), San Raffaele Scientific Institute, Vita-Salute San Raffaele University and Center for Translational Genomics and Bioinformatics, Centre de biophysique moléculaire (CBM), Université d'Orléans (UO)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC), Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA), Lugardon, K, Raffner, R, Goumon, Y, Corti, Angelo, Delmas, A, Bulet, P, Aunis, D, Metz Boutigue, Mh |
| Rok vydání: | 2000 |
| Předmět: |
endocrine system
Neutrophils [SDV]Life Sciences [q-bio] Molecular Sequence Data 030209 endocrinology & metabolism Peptide Biology Biochemistry law.invention Structure-Activity Relationship 03 medical and health sciences 0302 clinical medicine Immune system Anti-Infective Agents In vivo law Chromogranins Animals Humans Amino Acid Sequence Molecular Biology Peptide sequence 030304 developmental biology chemistry.chemical_classification 0303 health sciences Innate immune system Bacteria Fungi Chromogranin A Cell Biology Peptide Fragments Recombinant Proteins Anti-Bacterial Agents Rats chemistry Recombinant DNA biology.protein Cattle Antibacterial activity |
| Zdroj: | Journal of Biological Chemistry Journal of Biological Chemistry, American Society for Biochemistry and Molecular Biology, 2000, 275 (15), pp.10745-10753. ⟨10.1074/jbc.275.15.10745⟩ |
| ISSN: | 0021-9258 1083-351X |
| Popis: | International audience; Vasostatin-1, the natural N-terminal 1-76 chromogranin A (CGA)-derived fragment in bovine sequence, has been purified from chromaffin secretory granules and identified by sequencing and matrix-assisted laser desorption time-of-flight mass spectrometry. This peptide, which displays antibacterial activity against Gram-positive bacteria at micromolar concentrations, is also able to kill a large variety of filamentous fungi and yeast cells in the 1-10 microM range. We have found that the C-terminal moiety of vasostatin-1 is essential for the antifungal activity, and shorter active peptides have been synthesized. In addition, from the comparison with the activity displayed by related peptides (human recombinant and rat synthetic fragments), we could determine that antibacterial and antifungal activities have different structural requirements. To assess for such activities in vivo, CGA and CGA-derived fragments were identified in secretory material released from human polymorphonuclear neutrophils upon stimulation. Vasostatin-1, which is stored in a large variety of cells (endocrine, neuroendocrine, and neurons) and which is liberated from stimulated chromaffin and immune cells upon stress, may represent a new component active in innate immunity. |
| Databáze: | OpenAIRE |
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