A simple architecture with self-assembled monolayers to build immunosensors for detecting the pancreatic cancer biomarker CA19-9
| Autor: | Andrey Soares, Juliana C. Soares, Matias Eliseo Melendez, Valquiria da Cruz Rodrigues, Flavio M. Shimizu, José Humberto Tavares Guerreiro Fregnani, André Lopes Carvalho, Iram T. Awan, Osvaldo N. Oliveira, Angelo L. Gobbi, Rui Manuel Reis, Maria H. O. Piazzetta |
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| Přispěvatelé: | Universidade do Minho |
| Rok vydání: | 2018 |
| Předmět: |
CA-19-9 Antigen
endocrine system diseases Medicina Básica [Ciências Médicas] SENSORES BIOMÉDICOS Biosensing Techniques 02 engineering and technology 01 natural sciences Biochemistry Analytical Chemistry Blood serum Pancreatic cancer Monolayer Electrochemistry medicine Humans Environmental Chemistry Electrodes Spectroscopy Immunoassay Detection limit Science & Technology medicine.diagnostic_test Chemistry 010401 analytical chemistry Self-assembled monolayer 021001 nanoscience & nanotechnology medicine.disease digestive system diseases 3. Good health 0104 chemical sciences Dielectric spectroscopy Pancreatic Neoplasms Dielectric Spectroscopy Ciências Médicas::Medicina Básica CA19-9 Gold 0210 nano-technology Biomedical engineering |
| Zdroj: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP Repositório Científico de Acesso Aberto de Portugal Repositório Científico de Acesso Aberto de Portugal (RCAAP) instacron:RCAAP |
| Popis: | Accepted Manuscript The challenge of the early diagnosis of pancreatic cancer in routine clinical practice requires low-cost means of detection, and this may be achieved with immunosensors based on electrical or electrochemical principles. In this paper, we report a potentially low-cost immunosensor built with interdigitated gold electrodes coated with a self-assembled monolayer and a layer of anti-CA19-9 antibodies, which is capable of detecting the pancreatic cancer biomarker CA19-9 using electrical impedance spectroscopy. Due to specific, irreversible adsorption of CA19-9 onto its corresponding antibody, according to data from polarization-modulated infrared reflection absorption spectroscopy (PM-IRRAS), the immunosensor is highly sensitive and selective. It could detect CA19-9 in commercial samples with a limit of detection of 0.68 U mL−1, in addition to distinguishing between blood serum samples from patients with different concentrations of CA19-9. Furthermore, by treating the capacitance data with information visualization methods, we were able to verify the selectivity and robustness of the immunosensor with regard to false positives, as the samples containing higher CA19-9 concentrations, including those from tumor cells, could be distinguished from those with possible interferents. CAPES, FAPESP (Grant 2013/14262-7 and 2012/15543-7), CNPq (150985/2017-7), nBioNet network and Barretos Cancer Hospital for financial support info:eu-repo/semantics/publishedVersion |
| Databáze: | OpenAIRE |
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