Detection of Intracellular Parasites by Use of the CellaVision DM96 Analyzer during Routine Screening of Peripheral Blood Smears
| Autor: | Hung S. Luu, Erin McElvania TeKippe, Christopher D. Doern, Lori D. Racsa, Paul M. Southern, Rita M. Gander |
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| Rok vydání: | 2015 |
| Předmět: |
Microbiology (medical)
Pathology medicine.medical_specialty Erythrocytes Plasmodium malariae Parasitemia Sensitivity and Specificity parasitic diseases Parasitic Diseases medicine Animals Humans Malaria Falciparum Microscopy Blood Cells Hematologic Tests Routine screening biology Reproducibility of Results Gold standard (test) biology.organism_classification medicine.disease Plasmodium ovale Peripheral blood Red blood cell medicine.anatomical_structure Babesia Parasitology |
| Zdroj: | Journal of Clinical Microbiology. 53:167-171 |
| ISSN: | 1098-660X 0095-1137 |
| DOI: | 10.1128/jcm.01783-14 |
| Popis: | Conventional microscopy is the gold standard for malaria diagnosis. The CellaVision DM96 is a digital hematology analyzer that utilizes neural networks to locate, digitize, and preclassify leukocytes and characterize red blood cell morphology. This study compared the detection rates of Plasmodium and Babesia species on peripheral blood smears utilizing the CellaVision DM96 with the rates for a routine red blood cell morphology scan. A total of 281 slides were analyzed, consisting of 130 slides positive for Plasmodium or Babesia species and 151 negative controls. Slides were blinded, randomized, and analyzed by CellaVision and microscopy for red cell morphology scans. The technologists were blinded to prior identification results. The parasite detection rate was 73% (95/130) for CellaVision and 81% (105/130) for microscopy for positive samples. The interobserver agreement between CellaVision and microscopy was fair, as Cohen's kappa coefficient equaled 0.36. Pathologist review of CellaVision images identified an additional 15 slides with parasites, bringing the total number of detectable positive slides to 110 of 130 (85%). Plasmodium ovale had the lowest rate of detection at 56% (5 of 9); Plasmodium malariae and Babesia spp. had the highest rate of detection at 100% (3/3 and 6/6, respectively). The detection rate by CellaVision was 100% (23/23) when the parasitemia was ≥2.5%. The detection rate for |
| Databáze: | OpenAIRE |
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