Anti-oxidant and anti-inflammatory effects of auraptene on phytohemagglutinin (PHA)-induced inflammation in human lymphocytes
| Autor: | Reza Zargarani, Vafa Baradaran Rahimi, Vahid Reza Askari, Mostafa Boskabady, Mohammad Hossein Boskabady, Razieh Ghodsi |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Adult
Male Cell Survival T-Lymphocytes Inflammation Pharmacology In Vitro Techniques medicine.disease_cause Antioxidants Dexamethasone Superoxide dismutase 03 medical and health sciences chemistry.chemical_compound Young Adult 0302 clinical medicine Coumarins medicine Humans Viability assay Phytohemagglutinins chemistry.chemical_classification Reactive oxygen species biology General Medicine Glutathione chemistry 030220 oncology & carcinogenesis Auraptene biology.protein Cytokines Tumor necrosis factor alpha medicine.symptom Reactive Oxygen Species 030217 neurology & neurosurgery Oxidative stress |
| Zdroj: | Pharmacological reports : PR. 73(1) |
| ISSN: | 2299-5684 |
| Popis: | Inflammation is characterized as a defensive response of our body against endogenous or exogenous stimuli. Chronic inflammation and oxidative stress play an important role in the pathogenesis of various disorders such as asthma, cancers, and multiple sclerosis. Recently, diverse pharmacological activities of auraptene, a natural prenyloxycoumarin, were reported. In the present study, we aimed to evaluate the anti-oxidative and anti-inflammatory effects of auraptene on human isolated lymphocytes. The effects of auraptene (10, 30 and 90 μM) and dexamethasone (0.1 mM) were evaluated on cell viability, reactive oxygen species (ROS), and malondialdehyde (MDA) levels, superoxide dismutase (SOD) and catalase (CAT) activities, and total glutathione content (GSH) as well as the secretion of interleukin 6 (IL-6) and tumor necrosis factor (TNF)-α in phytohemagglutinin (PHA)-stimulated human lymphocytes. Auraptene (10–90 μM) did not affect lymphocytes' viability after 48 h incubation. PHA markedly elevated ROS, MDA, IL-6, and TNF-α levels, while diminished the GSH content, and CAT and SOD activities in human lymphocytes (p |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink