First detection of Cryptosporidium DNA in blood and cerebrospinal fluid of HIV-infected patients
| Autor: | Natalia Vittar, Jorge Néstor Velásquez, María Laura Pantano, Silvana Carnevale, Javier Ricart, Mónica Gabriela Nigro, Daniela della Paolera, Olga Figueiras, Osvaldo Germán Astudillo |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Adult
Male 0301 basic medicine BLOOD CIENCIAS MÉDICAS Y DE LA SALUD Cryptosporidium infection animal diseases 030231 tropical medicine 030106 microbiology Cryptosporidium DNA Ciencias de la Salud Cryptosporidiosis Cryptosporidium CSF HIV Infections Real-Time Polymerase Chain Reaction DNA Ribosomal Feces Immunocompromised Host 03 medical and health sciences 0302 clinical medicine Cerebrospinal fluid CRYPTOSPORIDIUM SP parasitic diseases Biopsy medicine Animals Humans Hiv infected patients Parasitología General Veterinary medicine.diagnostic_test biology General Medicine DNA Protozoan Middle Aged medicine.disease biology.organism_classification Virology Subtyping AIDS PCR Infectious Diseases Insect Science Parasitology Cryptosporidium hominis Polymorphism Restriction Fragment Length |
| Zdroj: | Parasitology Research. 117:875-881 |
| ISSN: | 1432-1955 0932-0113 |
| Popis: | Human cryptosporidiosis is an intestinal infection caused by different species belonging to the genus Cryptosporidium in both immunocompetent and immunocompromised individuals. The life cycle of Cryptosporidium sp. when affecting the digestive system is well known but the infection of other organs is less studied. Molecular methods are necessary for species and subtypes identification. The goal of this work is to propose a new approach that contributes to the diagnosis of the extra-intestinal dissemination process of Cryptosporidium infection. Cryptosporidium sp. was detected in stool and biopsy samples of two HIV-infected patients. DNA was extracted from feces, biopsy specimens, blood, and cerebrospinal fluid (CSF). All samples were analyzed by nested PCR-RFLP of the 18S rDNA, real-time PCR, and gp60 subtyping. Cryptosporidium DNA was detected in stool and tissue samples and it was also present in blood and CSF samples. Both cases were characterized as Cryptosporidium hominis subtype IeA11G3T3. This is the first report that demonstrates the presence of Cryptosporidium DNA in blood and CSF of HIV-infected patients. Fil: Velásquez, Jorge Néstor. Gobierno de la Ciudad de Buenos Aires. Hospital de Infecciosas "Dr. Francisco Javier Muñiz"; Argentina Fil: Pantano, María Laura. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud “Dr. C. G. Malbrán”; Argentina Fil: Vittar, Natalia. Gobierno de la Ciudad de Buenos Aires. Hospital de Infecciosas "Dr. Francisco Javier Muñiz"; Argentina Fil: Nigro, Mónica Gabriela. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud “Dr. C. G. Malbrán”; Argentina Fil: Figueiras, Olga. Gobierno de la Ciudad de Buenos Aires. Hospital de Infecciosas "Dr. Francisco Javier Muñiz"; Argentina Fil: Astudillo, Osvaldo Germán. Gobierno de la Ciudad de Buenos Aires. Hospital de Infecciosas "Dr. Francisco Javier Muñiz"; Argentina. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud “Dr. C. G. Malbrán”; Argentina Fil: Ricart, Javier. Gobierno de la Ciudad de Buenos Aires. Hospital de Infecciosas "Dr. Francisco Javier Muñiz"; Argentina Fil: della Paolera, Daniela. Gobierno de la Ciudad de Buenos Aires. Hospital de Infecciosas "Dr. Francisco Javier Muñiz"; Argentina Fil: Carnevale, Silvana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud “Dr. C. G. Malbrán”; Argentina |
| Databáze: | OpenAIRE |
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