On the evolution and physiology of cable bacteria
| Autor: | Markus Schmid, Tingting Yang, Lars Peter Nielsen, Thomas Boesen, Michael Wagner, Morten Simonsen Dueholm, Andreas Bøggild, Jesper Tataru Bjerg, Kasper Urup Kjeldsen, Marta Nierychlo, Per Halkjær Nielsen, Jeanine S. Geelhoed, Andreas Schramm, Lars Schreiber, Casper Thorup, Steffen Larsen, Filip J. R. Meysman, Jack van de Vossenberg, Nils Risgaard-Petersen |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Sulfide
microbial evolution microbial genome 03 medical and health sciences Electricity Gene family Cable bacteria 030304 developmental biology chemistry.chemical_classification 0303 health sciences Multidisciplinary biology Microbial physiology Bacteria 030306 microbiology Microbial evolution Periplasmic space Electromicrobiology biology.organism_classification cable bacteria chemistry Biochemistry PNAS Plus Metagenomics Microbial genome Horizontal gene transfer Metaproteomics Engineering sciences. Technology electromicrobiology microbial physiology Desulfobulbaceae |
| Zdroj: | Proceedings of the National Academy of Sciences of the United States of America Kjeldsen, K U, Schreiber, L, Thorup, C A, Boesen, T, Bjerg, J T, Yang, T, Dueholm, M S, Larsen, S, Risgaard-Petersen, N, Nierychlo, M, Schmid, M, Bøggild, A, Vossenberg, J V D, Geelhoed, J S, Meysman, F J R, Wagner, M, Nielsen, P H, Nielsen, L P & Schramm, A 2019, ' On the evolution and physiology of cable bacteria ', Proceedings of the National Academy of Sciences of the United States of America, vol. 116, no. 38, pp. 19116-19125 . https://doi.org/10.1073/pnas.1903514116 Kjeldsen, K U, Schreiber, L, Thorup, C A, Boesen, T, Bjerg, J T, Yang, T, Dueholm, M S, Larsen, S, Risgaard-Petersen, N, Nierychlo, M, Schmid, M, Bøggild, A, van de Vossenberg, J, Geelhoed, J S, Meysman, F J R, Wagner, M, Nielsen, P H, Nielsen, L P & Schramm, A 2019, ' On the evolution and physiology of cable bacteria ', Proceedings of the National Academy of Sciences, vol. 116, no. 38, pp. 19116-19125 . https://doi.org/10.1073/pnas.1903514116 |
| ISSN: | 0027-8424 |
| DOI: | 10.1073/pnas.1903514116 |
| Popis: | Cable bacteria of the family Desulfobulbaceae form centimeter-long filaments comprising thousands of cells. They occur worldwide in the surface of aquatic sediments, where they connect sulfide oxidation with oxygen or nitrate reduction via long-distance electron transport. In the absence of pure cultures, we used single-filament genomics and metagenomics to retrieve draft genomes of 3 marine Candidatus Electrothrix and 1 freshwater Ca. Electronema species. These genomes contain >50% unknown genes but still share their core genomic makeup with sulfate-reducing and sulfur-disproportionating Desulfobulbaceae, with few core genes lost and 212 unique genes (from 197 gene families) conserved among cable bacteria. Last common ancestor analysis indicates gene divergence and lateral gene transfer as equally important origins of these unique genes. With support from metaproteomics of a Ca. Electronema enrichment, the genomes suggest that cable bacteria oxidize sulfide by reversing the canonical sulfate reduction pathway and fix CO 2 using the Wood–Ljungdahl pathway. Cable bacteria show limited organotrophic potential, may assimilate smaller organic acids and alcohols, fix N 2 , and synthesize polyphosphates and polyglucose as storage compounds; several of these traits were confirmed by cell-level experimental analyses. We propose a model for electron flow from sulfide to oxygen that involves periplasmic cytochromes, yet-unidentified conductive periplasmic fibers, and periplasmic oxygen reduction. This model proposes that an active cable bacterium gains energy in the anodic, sulfide-oxidizing cells, whereas cells in the oxic zone flare off electrons through intense cathodic oxygen respiration without energy conservation; this peculiar form of multicellularity seems unparalleled in the microbial world. |
| Databáze: | OpenAIRE |
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