Custom fabrication and mode-locked operation of a femtosecond fiber laser for multiphoton microscopy
Autor: | Bryan Q. Spring, Guillaume Ducourthial, Nima Davoudzadeh |
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Jazyk: | angličtina |
Rok vydání: | 2019 |
Předmět: |
0301 basic medicine
Ytterbium Materials science Fabrication Optical fiber chemistry.chemical_element lcsh:Medicine Lasers Solid-State Article law.invention 03 medical and health sciences 0302 clinical medicine law Fiber laser Fusion splicing Fiber Optic Technology lcsh:Science Multidisciplinary business.industry lcsh:R Laser science Laser Microscopy Fluorescence Multiphoton 030104 developmental biology chemistry Femtosecond Optoelectronics lcsh:Q business 030217 neurology & neurosurgery |
Zdroj: | Scientific Reports, Vol 9, Iss 1, Pp 1-12 (2019) Scientific Reports |
ISSN: | 2045-2322 |
Popis: | Solid-state femtosecond lasers have stimulated the broad adoption of multiphoton microscopy in the modern laboratory. However, these devices remain costly. Fiber lasers offer promise as a means to inexpensively produce ultrashort pulses of light suitable for nonlinear microscopy in compact, robust and portable devices. Although encouraging, the initial methods reported in the biomedical engineering community to construct home-built femtosecond fiber laser systems overlooked fundamental aspects that compromised performance and misrepresented the significant financial and intellectual investments required to build these devices. Here, we present a practical protocol to fabricate an all-normal-dispersion ytterbium (Yb)-doped femtosecond fiber laser oscillator using commercially-available parts (plus standard optical components and extra-cavity accessories) as well as basic fiber splicing and laser pulse characterization equipment. We also provide a synthesis of established protocols in the laser physics community, but often overlooked in other fields, to verify true versus seemingly (partial or noise-like) mode-locked performance. The approaches described here make custom fabrication of femtosecond fiber lasers more accessible to a wide range of investigators and better represent the investments required for the proper laser design, fabrication and operation. |
Databáze: | OpenAIRE |
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