Heterologous production of the widely used natural food colorant carminic acid in Aspergillus nidulans

Autor:	Rasmus John Normand Frandsen, Dan Staerk, Paiman Khorsand-Jamal, Kim Binderup, Majse Nafisi, Rubini Maya Kannangara, Finn Thyge Okkels, Ulf Thrane, Kenneth T. Kongstad, Uffe Hasbro Mortensen, Bjørn Madsen, Birger Lindberg Møller
Jazyk:	angličtina
Rok vydání:	2018
Předmět:	0301 basic medicine Heterologous lcsh:Medicine 010402 general chemistry Carmine 01 natural sciences Anthrone Cyclase Aspergillus nidulans Article Catalysis Hemiptera 03 medical and health sciences chemistry.chemical_compound Animals Metabolomics lcsh:Science Biological Products Multidisciplinary biology Carminic acid lcsh:R Food Coloring Agents Monooxygenase biology.organism_classification Biosynthetic Pathways 0104 chemical sciences 030104 developmental biology chemistry Biochemistry Polyketides Metabolome Fermentation lcsh:Q
Zdroj:	Scientific Reports, Vol 8, Iss 1, Pp 1-10 (2018) Frandsen, R J N, Khorsand-Jamal, P, Kongstad, K T, Nafisi, M, Kannangara, R M, Stærk, D, Okkels, F T, Binderup, K, Madsen, B, Møller, B L, Thrane, U & Mortensen, U H 2018, ' Heterologous production of the widely used natural food colorant carminic acid in Aspergillus nidulans ', Scientific Reports, vol. 8, 12853, pp. 1-10 . https://doi.org/10.1038/s41598-018-30816-9 Frandsen, R J N, Khorsand-Jamal, P, Kongstad, K T, Nafisi, M, Kannangara, R M, Stærk, D, Okkels, F T, Binderup, K, Madsen, B, Møller, B L, Thrane, U & Mortensen, U H 2018, ' Heterologous production of the widely used natural food colorant carminic acid in Aspergillus nidulans ', Scientific Reports, vol. 8, no. 1 . https://doi.org/10.1038/s41598-018-30816-9 Scientific Reports Frandsen, R J N, Khorsand-Jamal, P, Kongstad, K T, Nafisi, M, Kannangara, R M, Staerk, D, Okkels, F T, Binderup, K, Madsen, B, Møller, B L, Thrane, U & Mortensen, U H 2018, ' Heterologous production of the widely used natural food colorant carminic acid in Aspergillus nidulans ', Scientific Reports, vol. 8, no. 1, 12853 . https://doi.org/10.1038/s41598-018-30816-9
ISSN:	2045-2322
DOI:	10.1038/s41598-018-30816-9
Popis:	The natural red food colorants carmine (E120) and carminic acid are currently produced from scale insects. The access to raw material is limited and current production is sensitive to fluctuation in weather conditions. A cheaper and more stable supply is therefore desirable. Here we present the first proof-of-concept of heterologous microbial production of carminic acid in Aspergillus nidulans by developing a semi-natural biosynthetic pathway. Formation of the tricyclic core of carminic acid is achieved via a two-step process wherein a plant type III polyketide synthase (PKS) forms a non-reduced linear octaketide, which subsequently is folded into the desired flavokermesic acid anthrone (FKA) structure by a cyclase and a aromatase from a bacterial type II PKS system. The formed FKA is oxidized to flavokermesic acid and kermesic acid, catalyzed by endogenous A. nidulans monooxygenases, and further converted to dcII and carminic acid by the Dactylopius coccus C-glucosyltransferase DcUGT2. The establishment of a functional biosynthetic carminic acid pathway in A. nidulans serves as an important step towards industrial-scale production of carminic acid via liquid-state fermentation using a microbial cell factory.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b6b9d0cfaad562d345b4c890db9614a9 http://link.springer.com/article/10.1038/s41598-018-30816-9 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
Nepřihlášeným uživatelům se plný text nezobrazuje	K zobrazení výsledku je třeba se přihlásit.