Fast, Sensitive, and Quantitative Point-of-Care Platform for the Assessment of Drugs of Abuse in Urine, Serum, and Whole Blood
| Autor: | Lidong Qin, Ping Wang, Bangshun He, Ying Li, Uvaraj Uddayasankar |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
Drug
medicine.medical_specialty Drugs of abuse Point-of-Care Systems media_common.quotation_subject 02 engineering and technology Urine Pharmacology Sensitivity and Specificity 01 natural sciences Article Analytical Chemistry Lab-On-A-Chip Devices medicine Humans Intensive care medicine Point of care media_common Whole blood Illicit Drugs Chemistry 010401 analytical chemistry Microfluidic Analytical Techniques 021001 nanoscience & nanotechnology medicine.disease Serum samples 0104 chemical sciences Substance abuse 0210 nano-technology |
| Zdroj: | Analytical Chemistry. 89:8273-8281 |
| ISSN: | 1520-6882 0003-2700 |
| DOI: | 10.1021/acs.analchem.7b01288 |
| Popis: | Drug abuse is a major public health problem in many countries in Europe and North America. Currently available platforms for drug abuse assessment are facing technical challenges of nonquantitation, inaccuracy, low throughput, incompatibility with diverse complex specimens, long assay times, and requirement of instrument and/or expertise for readout. Here, we report an integrated competitive volumetric-bar-chart chip (CV-Chip) to assay multiple drug targets at the point-of-care (POC). To the best of our knowledge, it is the first time that a POC platform has been demonstrated to fully address the above-mentioned limitations. We applied this integrated CV-chip platform to assay multiple drugs in 38 patient urine and serum samples and validated the on-chip results with an LC-MS/MS method, indicating a clinical sensitivity and specificity of 0.94 and 1.00, respectively. We further demonstrated that the combination of an on-chip blood separator with the CV-Chip enabled the platform to directly assay finger-prick whole blood samples, which have always been recognized as an ideal biospecimen for POC detections. In summary, this integrated CV-Chip is able to serve as a sensitive, accurate, fast, portable, readout visible, and minimally invasive platform for drug abuse assessment. |
| Databáze: | OpenAIRE |
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