Consistent expression of guanylyl cyclase-C in primary and metastatic gastrointestinal cancers
| Autor: | Feng Gao, Claudia Mescoli, Thea Kalebic, Massimo Rugge, William L. Trepicchio, Matteo Fassan, Tim Wyant, Hadi Danaee |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Colorectal cancer Adenocarcinoma Gastrointestinal Neoplasms Humans Neoplasm Metastasis Receptors Enterotoxin Protein Expression lcsh:Medicine Enterotoxin 0302 clinical medicine Membrane region Adenocarcinomas Receptors Medicine and Health Sciences lcsh:Science Receptor Staining Multidisciplinary Tissue microarray Squamous Cell Carcinomas Guanylate cyclase 2C Membrane Staining Oncology 030220 oncology & carcinogenesis Immunohistochemistry Research Article Research and Analysis Methods Carcinomas 03 medical and health sciences medicine Gene Expression and Vector Techniques Molecular Biology Techniques Cytoplasmic Staining Molecular Biology Colorectal Cancer Molecular Biology Assays and Analysis Techniques business.industry lcsh:R Cancer Cancers and Neoplasms Biology and Life Sciences medicine.disease Genitourinary Tract Tumors 030104 developmental biology Metastatic Tumors Tumor progression Specimen Preparation and Treatment Cancer research lcsh:Q business |
| Zdroj: | PLoS ONE PLoS ONE, Vol 12, Iss 12, p e0189953 (2017) |
| ISSN: | 1932-6203 |
| Popis: | Background The transmembrane receptor guanylate cyclase-C (GCC) has been found to be expressed in colorectal cancers. However, limited data are available on GCC protein expression in non-colorectal gastrointestinal tumors and few studies have reported whether GCC protein expression was consistently preserved in synchronous primary and metastatic cancer tissues. Methods GCC protein status was assessed by immunohistochemistry in tumor specimens from individuals (n = 627) with gastrointestinal tumors, including esophageal (n = 130), gastric (n = 276), pancreatic (n = 136), and colorectal (n = 85) primary and metastatic tumors. Tissue specimens consisted of tissue microarrays containing esophageal, gastric, pancreatic tumors, and whole-slide tissue sections from colorectal cancer patients with matching primary and metastatic tumors Result Among the evaluated esophageal, gastric, and pancreatic tumors, the frequency of GCC positivity at the protein level ranged from 59% to 68%. GCC was consistently expressed in primary and matched/synchronous metastatic lesions of colorectal cancer tissues derived from the same patients. Conclusion This observational study demonstrated the protein expression of GCC across various gastrointestinal malignancies. In all cancer histotypes, GCC protein localization was observed predominantly in the cytoplasm compared to the membrane region of tumor cells. Consistent immunohistochemistry detection of GCC protein expression in primary colorectal cancers and in their matched liver metastases suggests that the expression of GCC is maintained throughout the process of tumor progression and formation of metastatic disease. |
| Databáze: | OpenAIRE |
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