Cellular human CLE/C14orf166 protein interacts with influenza virus polymerase and is required for viral replication
| Autor: | Ariel Rodriguez, Amelia Nieto, Alicia Pérez-González |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
Hepatitis B virus DNA polymerase
viruses Immunology Molecular Sequence Data RNA-dependent RNA polymerase RNA polymerase II medicine.disease_cause Virus Replication Microbiology Virus Cell Line chemistry.chemical_compound VP40 Virology RNA polymerase Two-Hybrid System Techniques Protein Interaction Mapping Influenza A virus medicine Humans Polymerase biology Sequence Analysis DNA RNA-Dependent RNA Polymerase Molecular biology Genome Replication and Regulation of Viral Gene Expression chemistry Insect Science Host-Pathogen Interactions biology.protein Trans-Activators Protein Binding |
| Zdroj: | Journal of virology. 85(22) |
| ISSN: | 1098-5514 |
| Popis: | The influenza A virus polymerase associates with a number of cellular transcription-related factors, including RNA polymerase II. We previously described the interaction of influenza virus polymerase subunit PA with human CLE/C14orf166 protein (hCLE), a positive modulator of this cellular RNA polymerase. Here, we show that hCLE also interacts with the influenza virus polymerase complex and colocalizes with viral ribonucleoproteins. Silencing of hCLE causes reduction of viral polymerase activity, viral RNA transcription and replication, virus titer, and viral particle production. Altogether, these findings indicate that the cellular transcription factor hCLE is an important protein for influenza virus replication. |
| Databáze: | OpenAIRE |
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