Humanized bispecific antibody (mPEG × HER2) rapidly confers PEGylated nanoparticles tumor specificity for multimodality imaging in breast cancer
Autor: | En-Shuo Liu, Chiao-Yun Chen, I-Ju Chen, Yun-Chi Lu, Huei-Jen Chen, Fang-Ming Chen, Tung-Ho Wu, Yi-An Cheng, Rui-An Lin, Tian-Lu Cheng, Tzu-Yi Liao, Kuo-Hsiang Chuang, Yun-Ming Wang, Chih-Kuang Wang |
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Rok vydání: | 2020 |
Předmět: |
Bispecific antibody
PEGylated nanoparticle Receptor ErbB-2 Contrast Media Pharmaceutical Science Medicine (miscellaneous) 02 engineering and technology Multimodal Imaging 01 natural sciences Applied Microbiology and Biotechnology Polyethylene Glycols Anti-PEG antibody Drug Delivery Systems Antibodies Bispecific skin and connective tissue diseases Human Epidermal Growth Factor Receptor 2 Chemistry Immunogenicity 021001 nanoscience & nanotechnology Contrast agent lcsh:R855-855.5 MCF-7 Cells Molecular Medicine Female 0210 nano-technology Preclinical imaging medicine.drug Polyethylene glycol lcsh:Medical technology lcsh:Biotechnology Biomedical Engineering Breast Neoplasms Bioengineering 010402 general chemistry Breast cancer Cancer image Cell Line Tumor lcsh:TP248.13-248.65 medicine Humans Multimodality image Doxorubicin One-step formulation Tumor specificity neoplasms Research technology industry and agriculture medicine.disease Molecular medicine 0104 chemical sciences Cancer research Nanoparticles Pegylated nanoparticles |
Zdroj: | Journal of Nanobiotechnology, Vol 18, Iss 1, Pp 1-12 (2020) Journal of Nanobiotechnology |
ISSN: | 1477-3155 |
Popis: | Background Developing a universal strategy to improve the specificity and sensitivity of PEGylated nanoaparticles (PEG-NPs) for assisting in the diagnosis of tumors is important in multimodality imaging. Here, we developed the anti-methoxypolyethylene glycol (mPEG) bispecific antibody (BsAb; mPEG × HER2), which has dual specificity for mPEG and human epidermal growth factor receptor 2 (HER2), with a diverse array of PEG-NPs to confer nanoparticles with HER2 specificity and stronger intensity. Result We used a one-step formulation to rapidly modify the nanoprobes with mPEG × HER2 and optimized the modified ratio of BsAbs on several PEG-NPs (Lipo-DiR, SPIO, Qdot and AuNP). The αHER2/PEG-NPs could specifically target MCF7/HER2 cells (HER2++) but not MCF7/neo1 cells (HER2+/−). The αHER2/Lipo-DiR and αHER2/SPIO could enhance the sensitivity of untargeted PEG-NPs on MCF7/HER2 (HER2++). In in vivo imaging, αHER2/Lipo-DiR and αHER2/SPIO increased the specific targeting and enhanced PEG-NPs accumulation at 175% and 187% on 24 h, respectively, in HER2-overexpressing tumors. Conclusion mPEG × HER2, therefore, provided a simple one-step formulation to confer HER2-specific targeting and enhanced sensitivity and contrast intensity on HER2 positive tumors for multimodality imaging. |
Databáze: | OpenAIRE |
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