Humanized bispecific antibody (mPEG × HER2) rapidly confers PEGylated nanoparticles tumor specificity for multimodality imaging in breast cancer

Autor: En-Shuo Liu, Chiao-Yun Chen, I-Ju Chen, Yun-Chi Lu, Huei-Jen Chen, Fang-Ming Chen, Tung-Ho Wu, Yi-An Cheng, Rui-An Lin, Tian-Lu Cheng, Tzu-Yi Liao, Kuo-Hsiang Chuang, Yun-Ming Wang, Chih-Kuang Wang
Rok vydání: 2020
Předmět:
Bispecific antibody
PEGylated nanoparticle
Receptor
ErbB-2
Contrast Media
Pharmaceutical Science
Medicine (miscellaneous)
02 engineering and technology
Multimodal Imaging
01 natural sciences
Applied Microbiology and Biotechnology
Polyethylene Glycols
Anti-PEG antibody
Drug Delivery Systems
Antibodies
Bispecific
skin and connective tissue diseases
Human Epidermal Growth Factor Receptor 2
Chemistry
Immunogenicity
021001 nanoscience & nanotechnology
Contrast agent
lcsh:R855-855.5
MCF-7 Cells
Molecular Medicine
Female
0210 nano-technology
Preclinical imaging
medicine.drug
Polyethylene glycol
lcsh:Medical technology
lcsh:Biotechnology
Biomedical Engineering
Breast Neoplasms
Bioengineering
010402 general chemistry
Breast cancer
Cancer image
Cell Line
Tumor
lcsh:TP248.13-248.65
medicine
Humans
Multimodality image
Doxorubicin
One-step formulation
Tumor specificity
neoplasms
Research
technology
industry
and agriculture
medicine.disease
Molecular medicine
0104 chemical sciences
Cancer research
Nanoparticles
Pegylated nanoparticles
Zdroj: Journal of Nanobiotechnology, Vol 18, Iss 1, Pp 1-12 (2020)
Journal of Nanobiotechnology
ISSN: 1477-3155
Popis: Background Developing a universal strategy to improve the specificity and sensitivity of PEGylated nanoaparticles (PEG-NPs) for assisting in the diagnosis of tumors is important in multimodality imaging. Here, we developed the anti-methoxypolyethylene glycol (mPEG) bispecific antibody (BsAb; mPEG × HER2), which has dual specificity for mPEG and human epidermal growth factor receptor 2 (HER2), with a diverse array of PEG-NPs to confer nanoparticles with HER2 specificity and stronger intensity. Result We used a one-step formulation to rapidly modify the nanoprobes with mPEG × HER2 and optimized the modified ratio of BsAbs on several PEG-NPs (Lipo-DiR, SPIO, Qdot and AuNP). The αHER2/PEG-NPs could specifically target MCF7/HER2 cells (HER2++) but not MCF7/neo1 cells (HER2+/−). The αHER2/Lipo-DiR and αHER2/SPIO could enhance the sensitivity of untargeted PEG-NPs on MCF7/HER2 (HER2++). In in vivo imaging, αHER2/Lipo-DiR and αHER2/SPIO increased the specific targeting and enhanced PEG-NPs accumulation at 175% and 187% on 24 h, respectively, in HER2-overexpressing tumors. Conclusion mPEG × HER2, therefore, provided a simple one-step formulation to confer HER2-specific targeting and enhanced sensitivity and contrast intensity on HER2 positive tumors for multimodality imaging.
Databáze: OpenAIRE
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