Atomic Emission Method for Total Boron in Blood during Neutron-Capture Therapy
| Autor: | Jyrki Vähätalo, Markus Färkkilä, Inkeri Ruokonen, Martti Kulvik, Riitta Zilliacus, Juha Laakso, Merja Kallio |
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| Rok vydání: | 2001 |
| Předmět: |
Adult
Boron Compounds Male inorganic chemicals Phenylalanine Clinical Biochemistry Analytical chemistry chemistry.chemical_element Boron Neutron Capture Therapy Hematocrit Sensitivity and Specificity Mass Spectrometry 030218 nuclear medicine & medical imaging 03 medical and health sciences 0302 clinical medicine SDG 3 - Good Health and Well-being medicine Humans Irradiation Boron Inductively coupled plasma mass spectrometry Aged Whole blood medicine.diagnostic_test Chemistry Spectrophotometry Atomic Biochemistry (medical) Radiochemistry Atomic emission spectroscopy Middle Aged 030220 oncology & carcinogenesis Female Indicators and Reagents Inductively coupled plasma Quantitative analysis (chemistry) |
| Zdroj: | Europe PubMed Central Laakso, J, Kulvik, M, Ruokonen, I, Vähätalo, J, Zilliacus, R, Färkkilä, M & Kallio, M 2001, ' Atomic emission method for total boron in blood during neutron-capture therapy ', Clinical Chemistry, vol. 47, no. 10, pp. 1796-1803 . < http://clinchem.aaccjnls.org/content/clinchem/47/10/1796.full.pdf > |
| ISSN: | 1530-8561 0009-9147 |
| Popis: | Background: Boron neutron-capture therapy (BNCT) is a drug-targeted binary radiotherapy for cancer. The 10B capture of thermal neutrons induces secondary radiation within cells during irradiation. The most widely used boron carrier is 4-dihydroxyborylphenylalanine (BPA). The duration and timing of the irradiation is adjusted by monitoring 10B concentrations in whole blood. Methods: We developed a new method for boron determination that uses inductively coupled plasma atomic emission spectrometry (ICP-AES) and protein removal with trichloroacetic acid before analysis. This method was compared with the established but tedious inductively coupled plasma mass spectrometry (ICP-MS), which uses wet ashing as sample pretreatment. Erythrocyte boron concentrations were determined indirectly on the basis of plasma and whole blood boron concentrations and the hematocrit. The hematocrit was determined indirectly by measuring calcium concentrations in plasma and whole blood. Results: Within- and between-day CVs were Conclusions: The present method is feasible, accurate, and one of the fastest for boron determination during BNCT. Our results indicate that it is preferable to determine boron in plasma and in whole blood. Indirect erythrocyte-boron determination thus becomes possible and avoids the impact of preanalytical confounding factors, such as the influence of the hematocrit of the patient. Such an approach enables a more reliable estimation of the irradiation dose. |
| Databáze: | OpenAIRE |
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