A monoclonal antibody specific for Pseudomonas aeruginosa amidase
| Autor: | Carlos Novo, Alda Clemente, Amin Karmali, Paul Brown |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
medicine.drug_class
Immunology Blotting Western Monoclonal antibody medicine.disease_cause Microbiology Amidase Amidohydrolases Mice Antibody Specificity Genetics medicine Animals Mice Inbred BALB C biology Pseudomonas aeruginosa Chemistry Antibodies Monoclonal biology.organism_classification Chromatography Ion Exchange Molecular biology In vitro Blot Immunoglobulin M biology.protein Hybridoma technology Female Antibody Pseudomonadaceae |
| Zdroj: | Hybridoma. 20(4) |
| ISSN: | 0272-457X |
| Popis: | Amidase from Pseudomonas aeruginosa was purified by anionic exchange chromatography and used to immunise female Balb/c mice. Monoclonal antibodies (MAbs) were raised by hybridoma technology using Sp2/0 myeloma cells as fusion partner. A selected IgM subclass MAb was purified from in vitro hybridoma cell line supernatant by a two-step anionic exchange chromatography. The MAb was specific for amidase from P. aeruginosa as determined by Western blotting and recognized the native and denatured forms of the enzyme. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|