Short- and long-term impact of hyperoxia on the blood and retinal cells transcriptome in a mouse model of oxygen-induced retinopathy
| Autor: | Cecilie Revhaug, Teofila Książek, Jacek J Pietrzyk, Lars Oliver Baumbusch, Miroslaw Bik-Multanowski, Anne Gro W. Rognlien, Magdalena Zasada, Przemko Kwinta, Ola Didrik Saugstad, Katarzyna Szewczyk, Anna Madetko-Talowska, Agnieszka Grabowska |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Time Factors Hyperoxia Retinal Neovascularization Biology DEPTOR Peripheral blood mononuclear cell Retina Andrology Transcriptome 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Gene expression medicine Animals Retinopathy of Prematurity RNA Messenger Gene Expression Profiling Intracellular Signaling Peptides and Proteins Nuclear Proteins Retinal Retinopathy of prematurity medicine.disease Basic Science Article eye diseases Mice Inbred C57BL Gene expression profiling Disease Models Animal 030104 developmental biology Animals Newborn chemistry Pediatrics Perinatology and Child Health Leukocytes Mononuclear sense organs medicine.symptom 030217 neurology & neurosurgery |
| Zdroj: | Pediatric Research |
| ISSN: | 0031-3998 |
| Popis: | Background We aimed to identify global blood and retinal gene expression patterns in murine oxygen-induced retinopathy (OIR), a common model of retinopathy of prematurity, which may allow better understanding of the pathogenesis of this severe ocular prematurity complication and identification of potential blood biomarkers. Methods A total of 120 C57BL/6J mice were randomly divided into an OIR group, in which 7-day-old pups were maintained in 75% oxygen for 5 days, or a control group. RNA was extracted from the whole-blood mononuclear cells and retinal cells on days 12, 17, and 28. Gene expression in the RNA samples was evaluated with mouse gene expression microarrays. Results There were 38, 1370 and 111 genes, the expression of which differed between the OIR and control retinas on days 12, 17, and 28, respectively. Gene expression in the blood mononuclear cells was significantly altered only on day 17. Deptor and Nol4 genes showed reduced expression both in the blood and retinal cells on day 17. Conclusion There are sustained marked changes in the global pattern of gene expression in the OIR mice retinas. An altered expression of Deptor and Nol4 genes in the blood mononuclear cells requires further investigation as they may indicate retinal neovascularization. |
| Databáze: | OpenAIRE |
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