Metaproteomics Reveals Alteration of the Gut Microbiome in Weaned Piglets Due to the Ingestion of the Mycotoxins Deoxynivalenol and Zearalenone
| Autor: | Bertrand Grenier, Moritz Bünger, Veronika Nagl, Alina Kurz, Maximiliane Dippel, Evelyne Selberherr, Andrea Ladinig, Ursula Ruczizka, Johan S. Saenz, Jana Seifert |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Proteomics
Firmicutes Swine Health Toxicology and Mutagenesis Microbial metabolism deoxynivalenol gut microbiome Weaning Carbohydrate metabolism Toxicology medicine.disease_cause Article chemistry.chemical_compound Therapy Animals medicine Ingestion Animals Humans Food science Mycotoxin Zearalenone biology Toxin zearalenone Mycotoxins biology.organism_classification Gastrointestinal Microbiome Disease Models Animal chemistry Metaproteomics Medicine metaproteomics Female Trichothecenes |
| Zdroj: | Toxins Volume 13 Issue 8 Toxins, Vol 13, Iss 583, p 583 (2021) |
| ISSN: | 2072-6651 |
| Popis: | The ingestion of mycotoxins can cause adverse health effects and represents a severe health risk to humans and livestock. Even though several acute and chronic effects have been described, the effect on the gut metaproteome is scarcely known. For that reason, we used metaproteomics to evaluate the effect of the mycotoxins deoxynivalenol (DON) and zearalenone (ZEN) on the gut microbiome of 15 weaned piglets. Animals were fed for 28 days with feed contaminated with different concentrations of DON (DONlow: 870 μg DON/kg feed, DONhigh: 2493 μg DON/kg feed) or ZEN (ZENlow: 679 μg ZEN/kg feed, ZENhigh: 1623 μg ZEN/kg feed). Animals in the control group received uncontaminated feed. The gut metaproteome composition in the high toxin groups shifted compared to the control and low mycotoxin groups, and it was also more similar among high toxin groups. These changes were accompanied by the increase in peptides belonging to Actinobacteria and a decrease in peptides belonging to Firmicutes. Additionally, DONhigh and ZENhigh increased the abundance of proteins associated with the ribosomes and pentose-phosphate pathways, while decreasing glycolysis and other carbohydrate metabolism pathways. Moreover, DONhigh and ZENhigh increased the abundance of the antioxidant enzyme thioredoxin-dependent peroxiredoxin. In summary, the ingestion of DON and ZEN altered the abundance of different proteins associated with microbial metabolism, genetic processing, and oxidative stress response, triggering a disruption in the gut microbiome structure. |
| Databáze: | OpenAIRE |
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