Deletion of Prdm8 impairs development of upper-layer neocortical neurons
| Autor: | Mayuko Inoue, Hitoshi Miyachi, Mariko Komabayashi-Suzuki, Tae Komai, Aya Honda, Emiko Yamanishi, Waka Teshima, Ken-ichi Mizutani, Ryota Iwai, Satsuki Kitano, Chisato Watanabe, Kazuyuki Yamagata |
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| Rok vydání: | 2015 |
| Předmět: |
Mice
Knockout Neurons Genetics Candidate gene Neocortex Protein domain Histone-Lysine N-Methyltransferase Cell Biology Biology DNA-binding protein Homology (biology) Cell biology DNA-Binding Proteins Gene expression profiling Mice medicine.anatomical_structure Histone Methyltransferases medicine Animals Immunohistochemistry Gene Gene Deletion |
| Zdroj: | Genes to Cells. 20:758-770 |
| ISSN: | 1356-9597 |
| Popis: | Upper-layer (UL) neocortical neurons are the most prominent distinguishing features of the mammalian neocortex compared with those of the avian dorsal cortex and are vastly expanded in primates. However, little is known about the identities of the genes that control the specification of UL neurons. Here, we found that Prdm8, a member of the PR (PRDI-BF1 and RIZ homology) domain protein family, was specifically expressed in the postnatal UL neocortex, particular those in late-born RORß-positive layer IV neurons. We generated homozygous Prdm8 knockout (Prdm8 KO) mice and found that the deletion of Prdm8 causes growth retardation and a reduced brain weight, although the brain weight-to-body weight ratio is unchanged at postnatal day 8 (P8). Immunohistochemistry showed that the relative UL thickness, but not the thickness of the deep layer (DL), was significantly reduced in Prdm8 KO mice compared with wild-type (WT) mice. In addition, we found that a number of late-born Brn2-positive UL neurons were significantly decreased in Prdm8 KO mice. To identify genes regulated by Prdm8 during neocortical development, we compared expression profiling analysis in Prdm8 KO and WT mice, and identified some candidate genes. These results suggest that the proper expression of Prdm8 is required for the normal development and construction of UL neurons in the mammalian neocortex. |
| Databáze: | OpenAIRE |
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