The reaction of the mouse blastocyst and its zona pellucida to enzymes in vitro

Autor: Patricia Bowman, Anne McLaren
Rok vydání: 1970
Předmět:
Zdroj: Development. 24:331-334
ISSN: 1477-9129
0950-1991
DOI: 10.1242/dev.24.2.331
Popis: Mouse blastocysts were cultured for 24 h in the presence of various substances at concentrations as high as possible, consistent with blastocyst survival. Lysis of the zona pellucida was produced by trypsin and pronase, but not by hyaluronidase, collagenase, β-glucuroni-dase, ribonuclease, neuraminidase or oestrogen. This suggests that the zona lysin secreted by the mouse uterus at the time of implantation consists of a proteolytic enzyme.
Databáze: OpenAIRE