Comparison of different diagnostic tests in dogs uninfected and naturally infected with visceral leishmaniasis
| Autor: | Simone Baldini Lucheis, Helio Langoni, C. Y. Sassaki, Marcia Moleta Colodel, Isabelle Ferreira, F. S. Nogueira, Noeme Sousa Rocha |
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| Přispěvatelé: | Universidade Estadual Paulista (Unesp), Educational Foundation of Andradina (FEA), Agência Paulista de Tecnologia dos Agronegócios (APTA) |
| Rok vydání: | 2011 |
| Předmět: |
dogs
Pathology diagnosis Immunofluorescence Toxicology law.invention law Diagnosis lcsh:Zoology Dog visceral leishmaniasis Blood culture lcsh:QL1-991 Polymerase chain reaction Visceral leishmaniasis Leishmania medicine.diagnostic_test biology Diagnostic test Infectious Diseases Animal parasitosis Differential diagnosis medicine.medical_specialty lcsh:Arctic medicine. Tropical medicine lcsh:RC955-962 Lymph node biopsy Dogs lcsh:RA1190-1270 medicine Animalia Animal experiment lcsh:Toxicology. Poisons business.industry Leishmaniasis Canis familiaris Nonhuman medicine.disease biology.organism_classification Laboratory diagnosis Aspiration cytology Animal Science and Zoology Parasitology business Controlled study |
| Zdroj: | Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 17, Iss 3, Pp 348-352 (2011) Scopus Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP |
| ISSN: | 1678-9199 |
| Popis: | Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-27T11:26:00Z No. of bitstreams: 0Bitstream added on 2014-05-27T14:41:53Z : No. of bitstreams: 1 2-s2.0-80052735361.pdf: 174713 bytes, checksum: 4e84b0b678722c9620fd3eeb13d9d696 (MD5) Made available in DSpace on 2014-05-27T11:26:00Z (GMT). No. of bitstreams: 0 Previous issue date: 2011-09-19 Item merged in doublecheck by Felipe Arakaki (arakaki@reitoria.unesp.br) on 2015-12-14T11:50:00Z Item was identical to item(s): 72165, 13389 at handle(s): http://hdl.handle.net/11449/72683, http://hdl.handle.net/11449/13714 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Fort Dodge Animal Health Uninfected dogs (n = 10) and those naturally infected with leishmaniasis (n = 10) were subjected to several diagnostic tests, namely: hemoculture, polymerase chain reaction (PCR) of hemoculture, indirect immunofuorescence (RIFI), cytological examination of lymph node aspirate, culture of lymph node aspirate and PCR of lymph node aspirate. RIFI - followed by PCR of lymph node aspirate culture - presented more positive results in infected dogs than in uninfected ones. In infected animals, RIFI was more efective than PCR of lymph node aspirate culture. There was no statistical diference in positivity between RIFI and hemoculture; lymph node aspirate culture/cytological examination of lymph node aspirate and PCR of hemoculture; and between PCR of lymph node aspirate culture and PCR of hemoculture. All infected and uninfected animals had positive and negative results in at least one test. In conclusion, the association of several tests improves the efcacy of canine visceral leishmaniasis diagnosis. Department of Clinical Veterinary Medicine School of Veterinary Medicine and Animal Husbandry Sao Paulo State University (Universidade Estadual Paulista (UNESP)), Botucatu, Sao Paulo State School of Veterinary Medicine Educational Foundation of Andradina (FEA), Andradina, Sao Paulo State Sao Paulo Agency of Agribusiness Technology (APTA), Bauru, Sao Paulo State Department of Veterinary Hygiene and Public Health School of Veterinary Medicine and Animal Husbandry Sao Paulo State University (Universidade Estadual Paulista (UNESP)), Botucatu, Sao Paulo State Department of Clinical Veterinary Medicine School of Veterinary Medicine and Animal Husbandry Sao Paulo State University (Universidade Estadual Paulista (UNESP)), Botucatu, Sao Paulo State Department of Veterinary Hygiene and Public Health School of Veterinary Medicine and Animal Husbandry Sao Paulo State University (Universidade Estadual Paulista (UNESP)), Botucatu, Sao Paulo State FAPESP: 2005/56765-9 |
| Databáze: | OpenAIRE |
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