Toxin-antigen conjugates as selection tools for antibody producing cells
| Autor: | Katrin Messerschmidt, Katja Heilmann |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Cell Survival
Ovalbumin medicine.drug_class Immunology medicine.disease_cause Monoclonal antibody Antibodies Mice chemistry.chemical_compound Antigen medicine Animals Immunology and Allergy Antigens Fluorescein Antibody-Producing Cells Screening procedures Institut für Biochemie und Biologie Mice Inbred BALB C Hybridomas Dose-Response Relationship Drug biology Chemistry Toxin Immunotoxins Antibodies Monoclonal Reproducibility of Results Fluoresceins Molecular biology eye diseases Methotrexate Immunologic Techniques biology.protein Hybridoma technology Antibody Conjugate |
| Popis: | The generation of antibodies with designated specificity requires cost-intensive and time-consuming screening procedures. Here we present a new method by which hybridoma cells can be selected based on the specificity of the produced antibody by the use of antigen-toxin-conjugates thus eliminating the need of a screening procedure. Initial experiments were done with methotrexate as low molecular weight toxin and fluorescein as model antigen. Methotrexate and a methotrexate-fluorescein conjugate were characterized regarding their toxicity. Afterwards the effect of the fluorescein-specific antibody B13-DE1 on the toxicity of the methotrexate-fluorescein conjugate was determined. Finally, first results showed that hybridoma cells that produce fluorescein specific antibodies are able to grow in the presence of fluorescein-toxin-conjugates. |
| Databáze: | OpenAIRE |
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